罗布麻叶总黄酮抗抑郁作用参与NE能系统可能机制的研究  

Study on the antidepressant-like effect and NE mechanisms of flavonoids extracted from Apocynum venetum leaves

作　　者：郑梅竹[1] 时东方[1] 范亚军[2] 刘春明[1] 

机构地区：[1]长春师范学院中心实验室,吉林长春130032 [2]长春师范学院生命科学院,吉林长春130032

出　　处：《广东农业科学》2012年第16期169-171,共3页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(30970299);吉林省科技厅重点项目(20090936);吉林省教育厅重点项目(2012]D228);长春师范学院自然科学基金(2012D004)

摘　　要：采用抗抑郁模型强迫游泳试验对罗布麻叶总黄酮的抗抑郁活性进行评价,同时选择相关NE受体阻断剂(α1-NE受体阻断剂→哌唑嗪、α2-NE受体阻断剂→育亨宾、αβ-NE受体阻断剂→普萘洛尔),采用小鼠悬尾试验探讨罗布麻叶总黄酮的抗抑郁机制。结果表明,罗布麻叶总黄酮具有抗抑郁作用;同时,与罗布麻叶总黄酮50 mg/kg单独给药相比α1、α2、αβ受体阻断剂哌唑嗪(1 mg/kg)、育亨宾(1 mg/kg)、普萘洛尔(2 mg/kg)与罗布麻叶总黄酮50 mg/kg联合连续灌胃给药10 d后,能明显延长悬尾小鼠累计不动时间,表明罗布麻叶总黄酮的抗抑郁活性与去甲肾上腺素能系统(α1、α2、αβ受体)有关。In this study, the classical animal models of depression including the tail suspension and forced swimming of the mouse was applied to evaluate the antidepressant-like effect of AV-extract. To further search for the neurotransmitter mechanism of antidepression of AV-extract, additionally, the monoaminergic mechanisms involved in the antidepressant-like effect of AV-extract in mice were also assessed. The results showed that AV-extract showed a clear antidepressant-like effect in variety of animal models of depression. The anti-immobility effect of AV-extract （50 mg/kg, p.o.） was completely prevented by the pretreatment of mice with prazosin （1 mg/kg, i.g., an α1-adrenoeeptor antagonist）, yohimbine （1 mg/kg, i.g., an α2-adrenoeeptor antagonist） or propranolol （2 mg/kg, i.g., α1- adrenoceptor antagonist）, Taken together, the data demonstrated that AV-extract produced an antidepressant-like that seems to be dependent on its interaction with the noradrenergic（α1-adrenoceptor, α2-adrenoceptor, αβ-adrenoceptor） systems.

关 键 词：罗布麻 抗抑郁 机制 

分 类 号：R285.5[医药卫生—中药学]