消癌解毒方含药血清诱导人肝癌SMMC-7721细胞凋亡的实验研究  

Apoptosis of Human Hepatoma SMMC-7721 Cells Induced by Xiao'ai Jiedu Prescription

作　　者：李黎[1] 王明艳[1] 吴勉华[1] 

机构地区：[1]南京中医药大学,江苏南京210046

出　　处：《时珍国医国药》2012年第8期1851-1853,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金项目(No.30973742/C190201);江苏省中医药局科技项目(No.LB09032)

摘　　要：目的探讨消癌解毒方的抗肿瘤作用机理。方法消癌解毒方含药血清作用人肝癌SMMC-7721细胞后,采用电镜技术观察细胞的形态改变,流式细胞仪Annexin V-FITC法检测细胞凋亡情况。结果消癌解毒方能明显促进肝癌细胞的凋亡。电镜下SMMC-7721细胞出现明显的细胞凋亡的形态学改变;凋亡作用以高剂量组效果最佳,凋亡率与顺铂组相似(P>0.05)。结论消癌解毒方通过诱导人肝癌SMMC-7721细胞凋亡抑制其生长。Objective To investigate the anticancer effect and mechanism of Xiao＇ai Jiedu Prescription（XJP）. Methods Human hepatoma SMMC -7721 cells were cultured together with pharmacological serum of XJP. Electron microscope and flow cytometry were used to detect cell apoptosis. Results It was shown that pharmacological serum of XJP could promote apoptosis of human hepatoma SMMC - 7721 cells. Cells showed significant apoptosis morphological changes under electron microscope. The apoptosis effect of large - dosage XJR group was the best, similar to that of chemotherapeutics （ P 〉 0.05 ）. Conclusion Pharmacological serum of XJP can inhibit the proliferation of SMMC -7721 cells by inducing apoptosis.

关 键 词：消癌解毒方 SMMC-7721细胞 凋亡 

分 类 号：R285.5[医药卫生—中药学]