表没食子儿茶素没食子酸酯对裸鼠肝癌移植瘤细胞凋亡作用的研究  

The-Epigallocatechin Gallate and Adriamycin Apoptosis of BEL-7404/ADR in vivo

作　　者：欧冰凝[1] 林晓贞[1] 梁钢[1] 孙悦文[1] 

机构地区：[1]广西医科大学药学院,广西南宁530021

出　　处：《时珍国医国药》2012年第8期1854-1855,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30760310);广西壮族自治区自然科学基金(No.2010GXNSFD013043);广西医学科学实验中心开放基金(No.KFJJ2011-38)

摘　　要：目的了解表没食子儿茶素没食子酸酯(EGCG)对裸鼠肝癌移植瘤细胞凋亡的影响。方法建立BEL-7404/ADR细胞裸鼠移植瘤模型,分为control组、ADM组、EGCG组、ADM联合低剂量EGCG组(AE low组)、ADM联合中剂量EGCG组(AE mid组)和ADM联合高剂量EGCG组(AE high组),给药20 d,处死裸鼠去瘤块。用免疫组织化学法(TUNEL法)检测瘤组织内原位细胞凋亡和流式细胞仪(FACS)检测肿瘤组织细胞周期。结果 ADM与低、中、高剂量的EGCG合用,其细胞凋亡率分别为16.7%,20.3%和25.9%,高于单用ADM组的9.3%(P<0.01),使肿瘤细胞增殖阻滞在S/G2期。结论 EGCG可协同增强ADM促肝癌MDR细胞BEL-7404/ADR的凋亡,使肿瘤细胞增殖周期阻滞在S/G2期。Objective To study on the -epigallocatechin gallate and adriamycin （ADM） apoptosis of BEL -7404/ ADR in vivo. Methods The model of xenografled tumor in nude mice was established with BEL - 7404/Adr. The experiment was divided into control group, ADM group, EGCG group and low, middle, high AE groups and received the desired drugs and dosage for 20 days. The apoptosis of BEL -7404/ ADR cells was detected by TUNEL method and the cell cycle was tested by FACS. Results The rates of cell apoptosis was 16.7% low AE group in 20.3% middle AE group and 25.9% in high AE group,and were all in higher than that of ADM group （9.3%） （ P 〈0.01 ）. EGCG combined with ADM arrested cells in S/G2 phase of the cell cycle in a dose - dependent manner. Conclusion EGCG combined with ADM can induce the apoptosis of BEL - 7404/ADR cells and block them in S/G： phase.

关 键 词：EGCG 肝癌细胞 凋亡 体内 

分 类 号：R285.5[医药卫生—中药学]