红花药材中红花黄色素含量的测定  

Determination of the Saflor Yellow-A in Carthamin tinctonus

作　　者：祝明[1] 郭增喜[1] 

机构地区：[1]浙江省药品检验所,杭州310004

出　　处：《中药材》2000年第8期458-459,共2页Journal of Chinese Medicinal Materials

摘　　要：建立了用高效液相色谱法测定红花药材中红花黄色素含量的方法。以十八烷基硅烷键合硅胶为固定相,甲醇一乙腈一0.7％磷酸水溶液(26:2:72)为流动相,流速为1.0ml/min,紫外检测波长为40nm。结果红花黄色素在0.52～4.16 μg范围内呈良好线性关系,回收率为 100.72％,RSD为 1.73％。The content of the sailor yellow-A in Carthamin tinctorius medicinal materials was determined by the HPLC method The C18-ODS( 150×4.5 mm) column and methanol-acetonitrile-0.7% H3PO4 (26:2:72) as a mobile phase were used.The flow rate was 1.0 ml/min and the detective wavelength was 403 nm. The average recovery was 100.72% and the relative standard deviation (RSD) was 1.73% .

关 键 词：红花 红花黄色素 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R282.71[医药卫生—中医学]