格列本脲对糖尿病及正常大鼠心肌磺脲类药物受体mRNA的影响  

Effects of glibenclamide on mRNA level of ATP-sensitive potassium channels of heart in normal and streptozotocin induced diabetic rats

作　　者：李焱[1] 邓庆丽[1] 傅祖植[1] 黎锋[1] 刘顺莲[1] 程桦[1] 

机构地区：[1]中山医科大学孙逸仙纪念医院内分泌科,广州510120

出　　处：《中华医学杂志》2000年第7期538-540,共3页National Medical Journal of China

基　　金：广东省科学技术委员会重点攻关课题基金资助项目;广东省卫生科学技术研究基金资助项目

摘　　要：目的　观察链脲霉素诱导的糖尿病大鼠的心肌磺脲类药物受体 (SUR1,SUR2 )和Kir6 .2是否在mRNA水平发生变化 ,并探讨格列本脲治疗对正常及糖尿病大鼠心肌SUR1,SUR2和Kir6 .2数量的影响。方法　成年雄性SD大鼠随机分为 3组 ,糖尿病组、糖尿病胰岛素治疗组和正常组。另外一部分成年雄性SD大鼠随机分为糖尿病格列本脲治疗组和糖尿病对照组 ,非糖尿病格列本脲治疗组和非糖尿病对照组。格列本脲治疗组按 10mg/kg体重的剂量腹腔注射格列本脲 2周。RT PCR生产地高辛标记的单链cDNA探针 ,斑点印迹杂交检测心肌SUR1、SUR2、Kir6 .2的mRNA含量变化。结果　糖尿病组、糖尿病胰岛素治疗组和正常对照组的心肌SUR1,SUR2 ,Kir6 .2的mRNA水平无差别 (P>0 0 5 ) ;格列本脲使非糖尿病大鼠心肌SUR1和SUR2的mRNA水平升高 (P <0 0 5 ) ,Kir6 .2的mRNA水平无改变 (P >0 0 5 )。格列本脲不影响糖尿病大鼠心肌SUR1、SUR2和Kir6 .2mRNA水平 (P >0 0 5 )。结论　链脲霉素诱导的实验性糖尿病不影响心肌SUR1、SUR2和Kir6 .2的mRNA水平 ,但改变心肌SUR1。Objective To estimate if any difference exists in mRNA expression levels of KATP (SUR1, SUR2, Kir6.2) in heart tissues between streptozotocin induced diabetic and normal rats, and to examine if any changes of heart KATP in mRNA level occurred after long term Glibenclamide administration among the diabetic and control rats. Methods Part 1: Adult male Sprague Dawley (SD) rats were allocated into three groups: one group ( n =13) was taken as normal control, and the other two groups were diabetic models induced by a single intraperitoneal injection of streptozotocin (60 mg/kg). One of the diabetic groups ( n =10) was treated with premixed insulin (30%RI, 70%NPH) 4 8 units/day by subcutaneous injection at 4 5 pm everyday for two weeks. Part 2: Four groups of adult male SD rats were enrolled into the study. Group one (D1) was diabetes control induced by streptozotocin ( n =9), group two (DG2) was the diabetes treated with Glibenclamide ( n =14). Group three (N3) was normal control ( n =10), and group four (NG4) received Glibenclamide administration without diabetes ( n =14). DG2 and NG4 were given 10 mg/kg Glibenclamide intraperitonealy at 4 5pm everyday for 14 days. Single stranded digoxigenin labeled probes were generated with RT PCR. A total of 30 μg of unfractionated RNA was transferred onto nylon membranes by dot blot. Hybridization was performed at 50℃ for 16 hours with high SDS concentration hybridization buffer. Results Study one: There was no significant difference in mRNA expression levels of SUR1, SUR2, and Kir6.2 in heart between diabetic, insulin treated diabetic and control groups ( P >0.05). Study two: Glibenclamide treated non diabetic rats had higher mRNA expression levels of SUR1 and SUR2 in heart than normal control. The SUR1 were 43.0±16.6 vs 30.8±7.8 ( P <0.05), SUR2 161.9±51.0 vs 118.9±40.9 ( P <0.05), respectively. No difference in heart Kir6.2 mRNA level was found between the two groups ( P >0.05). Comparison between Glibenclamide treated diabetic and

关 键 词：格列本脲 糖尿病 药物疗法 心肌磺脲类药物受体 

分 类 号：R587.1[医药卫生—内分泌]