转TaEBP基因小麦的回交转育研究  

Tansfer of TaEBP Gene Into Cultivated Wheat Variety by Backcrossing

作　　者：闵东红[1] 吴平 张小红[3] 徐兆师[4] 陈明[4] 李连城[4] 马有志[4] 

机构地区：[1]西北农林科技大学农学院/旱区作物逆境生物学国家重点实验室,陕西杨凌712100 [2]宁夏回族自治区永宁县农业技术推广服务中心,宁夏永宁750100 [3]西北农林科技大学生命科学学院,陕西杨凌712100 [4]中国农业科学院作物科学研究所/国家农作物基因资源和基因改良重大科学工程/农业部作物遗传改良与育种重点开放实验室,北京100081

出　　处：《种子》2012年第8期18-22,26,共6页Seed

基　　金：转基因生物新品种培育科技重大专项(编号:2009 ZX 08002-008 B);西北农林科技大学唐仲英育种基金

摘　　要：本研究以转TaEBP基因的新春9号小麦T 4代株系为供体,通过回交育种的方法将与抗逆相关的TaEBP转录因子基因导入黄淮麦区的7个主栽或主推小麦品种中,利用温室快速加代和目的基因PCR分子跟踪检测技术,实现了一年四代的快速繁育,在一年内获得了转基因回交三代株系群。PCR分析结果显示,TaEBP基因在杂交F1代植株中阳性率平均在72.3%以上,在6个轮回亲本的回交后代中基本符合1∶1分离比率,在刑麦六号的BC3F2代中呈3∶1分离,外源TaEBP基因的分布符合一对显性等位基因扩增结果的遗传分离比例;PEG-6000胁迫条件下,对3个来自刑麦6号BC3F2代的回交导入系苗期抗性鉴定显示,3个转基因回交导入系的抗旱性明显提高。表明利用转基因材料通过回交转育、快速加代和分子跟踪检测是快速定向改良小麦品种的性状、获得新的转基因小麦的一条有效途径。Back-cross breeding combined with rapid generation-adding and target gene PCR molecular tracking detection was taken to transfer TaEBP genes to 7 wheat cultivars which are now widely used in Huang-huai wheat producing area. The transgenic BC3 Fl strain groups were obtained within one year. PCR analysis showed that the average positive rate of TaEBP gene in F1 hybrid was more than 72.3%, in the different backcross generation of 6 recurrent parents, the segregation ratio of genetic distribution of PCR-positive individual plants and PCR-negative indiVidual plants was about 1： 1, the ratio was 3：1 in BC3 F2 generation of Xingmai 6. The results showed that the distribution of exogenous TaEBP gene with a dominant allele amplification results of genetic ratio. Under PEG-6000 stress, the 3 interogression lines from Xingmai 6 BC3 F2 generations of baekcross introgression lines seedling resistance identification showed, the 3 transgenic introgression lines drought resistance increased obviously. The results confirmed that to transfer TaEBP gene into other wheat cuhivars by back-crossing,rapid generation-adding and target gene PCR molecular tracking detection is a simple and effective approach to getting a new transgenic wheat and directional improving resistance of wheat varieties.

关 键 词：TaEBP基因 快速定向育种 分子跟踪检测 生理抗性 

分 类 号：S512.1[农业科学—作物学]