新型聚乙烯亚胺衍生物在Hep G2细胞中转染和毒性的研究  

Transfection and Cytotoxicity Studies of Novel Polyethyleneimine Derivative in Hep G2 cells

作　　者：王菲[1] 吕楠[1] 张奇昕[1] 苏靖[1] 

机构地区：[1]上海交通大学药学院,上海200240

出　　处：《现代生物医学进展》2012年第22期4205-4207,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81001416)

摘　　要：目的:研究以对苯二甲醛(Terephthalaldehyde)为连接剂的聚乙烯亚胺(Polyethyleneimine,PEI)衍生物PEI-Tp对肝癌细胞Hep G2的转染活性和细胞毒性的影响。方法:以荧光素酶质粒作为报告基因,研究高分子和DNA的复合物在Hep G2细胞中的转染活性,用MTT的方法研究高分子对Hep G2细胞的毒性。结果:Hep G2细胞转染结果显示构建的聚乙烯亚胺衍生物PEI-Tp具有高效输送质粒的能力;细胞毒性结果显示PEI-Tp随着浓度的增加,其毒性显著低于PEI25 kDa。结论:Hep G2细胞实验数据显示PEI-Tp是一种高效、低毒,在基因治疗领域有相当前景的非病毒载体。Objective：To investigate the transfection efficiency and cell cytotoxicity of Polyethyleneimine（PEI） derivative linking with Terephthalaldehyde.Methods：Luciferase plasmid was used as the reporter gene to investigate transfection efficiency in Hep G2 cells.Cytotoxicity was detected by MTT method.Results：Hep G2 cells transfection and cytotoxicity results suggested that the gene carrier（PEI-Tp） expressed equal transfection efficiency with PEI 25kDa and Lipofectamine 2000,and had less cytotoxicity than that of PEI 25kDa.Conclusion：PEI-Tp could be a highly efficient and less toxic gene carrier which was promising in gene therapy.

关 键 词：聚乙烯亚胺衍生物 HEP G2细胞 转染 细胞毒性 

分 类 号：R971[医药卫生—药品]