穿山龙血清指纹图谱分析  

Studies on Chromatography Fingerprint of Dioscorea nipponica Serum

作　　者：于书仪[1] 季芳茹[1] 于栋华[2] 刘树民[2] 

机构地区：[1]佳木斯大学附属第一医院,黑龙江佳木斯154002 [2]黑龙江中医药大学中医药研究院,哈尔滨150040

出　　处：《中国实验方剂学杂志》2012年第17期80-82,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81173618);黑龙江省青年基金项目(QC2009C046);黑龙江卫生厅科研项目(2011-386)

摘　　要：目的:建立穿山龙大鼠体内HPLC血清指纹图谱。方法:应用Diamonsil C18柱(4.6 mm×250 mm,5μm),0.1%磷酸水-乙腈进行梯度洗脱,流速0.8 mL.min-1,检测波长210 nm,柱温25℃。结果:精密度、重复性、稳定性试验中共有峰相对峰面积、相对保留时间的RSD均<5.0%;结论:该方法简便、实用、可靠,为继续深入对穿山龙药效物质基础研究、药效作用机制研究等打下了坚实的基础。Objective： To establish a method for Dioscorea nipponica rats serum HPLC fingerprinting. Method： The separation was performed on a Diamonsil Cls column （4.6mm×250mm,5μm） with 0. 1% phosphoric acid water and acetonitrile as mobile phase by gradient elution; the flow rate was 0. 8 mL·min-l; detection wavelength was set at 254 nm; the column temperature was maintained at 25℃. Result： The RSD of precision, reproducibility, stability test of relative peak area and relative retention time was less than 5%. Conclusion： The method is simple, practical, reliable, which would lay a solid foundation for further studies on pharmacodynamic material basis of Dioscorea nipponica and pharmacodynamic action mechanism research.

关 键 词：穿山龙 反相高效液相色谱 血清指纹图谱 

分 类 号：R284.1[医药卫生—中药学]