北豆根的HPLC指纹图谱研究  

Research on HPLC Fingerprint of Rahizoma Menispermi

作　　者：李艳荣[1] 王领弟[1] 张晓峰[1] 潘海峰[1] 

机构地区：[1]承德医学院河北省中药研究与开发重点实验室,河北承德067000

出　　处：《中国实验方剂学杂志》2012年第17期127-130,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：2010年承德市科学技术研究与发展计划项目(20101312)

摘　　要：目的:建立同时测定承德产及其他主产区北豆根中蝙蝠葛苏林碱和蝙蝠葛碱的含量及其HPLC-UV指纹图谱。方法:采用ZORBAX SB-C18(4.6 mm×250 mm,5μm)色谱柱,乙腈和-0.5%甲酸水溶剂系统梯度洗脱,检测波长为268 nm,流速1.0 mL.min-1,进样量10μL。结果:蝙蝠葛苏林碱和蝙蝠葛碱的线性范围分别为0.072～2.29 g.L-1(r=0.999 6),0.053～1.70 g.L-1(r=0.999 9);平均回收率分别为98.7%(RSD 1.5%),100.6%(RSD 1.9%);确立了承德产北豆根的指纹图谱共有9个共有峰,其他主产区的指纹图谱共有13个共有峰。指纹图谱方法重复性、精密度良好,承德产5批北豆根(原药材)除2号样品为0.404外其他样品均>0.9;其他主产区12批北豆根(饮片)除9号样品为0.716外其他样品均>0.9。结论:方法重复性好,专属性强,为北豆根药材的质量控制提供科学依据。Objective： To develop a RP-HPLC method for the quantitative analysis of daurisoline and dauricine, and establish an HPLC-UV fingerprint of Rahizoma Menispermi. Method ： The alkaloids were separated on a ZORBAX SB-Cls （4.6 mm ×250 mm, 5μm） with gradient elution using acetonitrile and H2O-formic acid （100：0.5）solvent system and detected at 268 nm. Flow rate was 1.0 mL·min-1 and 10μL was injected every time. Result： The linear range of daurisoline and dauricine was 0. 072-2.29 mg·mL-1 （r =0. 999 6）, 0. 053- 1.70 mg·mL-1 （r = 0. 999 9） , respectively. The average recovery rates of daurisoline and dauricine were 98.7% （RSD 1.5% ）, 100.6% （RSD 1.9% ）, respectively. In the fingerprint, 9 common peaks of Rahizoma Menispermi from Chengde and 13 common peaks of Rahizoma Menispermi from other production areas were confirmed. The accuracy and reproducibility of the method of fingerprint were good. The results of similarity analysis were above 0.9 except No. 2 was 0. 404 of Rahizoma Menispermi from Chengde and No. 9 was 0. 716 from other production areas. Conclusion： The method can be used scientifically to evaluate the quality of the Rahizoma Menispermi qualitatively and quantitatively.

关 键 词：北豆根 蝙蝠葛苏林碱 蝙蝠葛碱 高效液相色谱法 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]