大黄炮制品各组分泻下作用的比较研究  

Comparative Study on Purgative Effects Among Different Components of Processed Rhez Radix et Rhizoma

作　　者：刘亮亮[1,2] 隋峰[2] 闫美娟[2] 李燕[2] 林娜[2] 郑燕芳[2] 肖永庆[2] 李丽[2] 

机构地区：[1]江西中医学院,南昌330004 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2012年第17期161-165,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金重点项目(30730111)

摘　　要：目的:分析比较大黄不同炮制品各组分的泻下作用及机制。方法:小鼠随机分为对照组和大黄各炮制品组。除对照组外,各给药组均给与相应组分生药剂量10 g.kg-1,观察5 h内各组泻下只数、各小鼠首次泻下时间、5 h内泻下次数和总排便次数;采用炭末推进法,研究各大黄炮制品对小鼠炭末推进的影响;大鼠随机分成13组,除对照组外,其余按生药剂量7 g.kg-1连续给药5 d,取结肠组织测定Na+-K+-ATP酶的活性。结果:生大黄组分2和生大黄组分3产生泻下作用,其余组别未见泻下作用;从总排便次数来看,除熟大黄组分2、组分3以及生大黄组分4和大黄炭组分4外,其他各组分均明显高于空白组(P<0.05或P<0.01);与生大黄组分2相比,熟大黄组分2的总排便次数显著减少(P<0.05);与生大黄组分3比较,熟大黄组分3和大黄炭组分3两组的总排便次数明显减少(P<0.01);大黄炮制品各组分除熟大黄组分2外,均有提高小鼠小肠炭末推进率的作用趋势,生大黄组分2和组分3小鼠小肠推进率与空白组比较具有显著性差异(P<0.05);各炮制品组分之间比较,生大黄组分2炭末推进率显著高于熟大黄组分2(P<0.05);生大黄组分3炭末推进率显著高于熟大黄组分3(P<0.05);大黄炮制品各组分均对大鼠结肠Na+-K+-ATP酶的活性有抑制作用,其中生大黄组分2,生大黄组分3,大黄炭组分3与空白对照组比较有显著差异(P<0.05或P<0.01)。组分2各炮制品之间,生大黄组分2抑制作用最强,与熟大黄和大黄炭相比差异显著(P<0.05或P<0.01);组分3各炮制品之间,生大黄组分3的活性低于其他两组,其中与熟大黄相比差异显著(P<0.05)。结论:在所有大黄各炮制品组分中,生大黄组分2和组分3的泻下作用最强,其作用机制可能与抑制Na+-K+-ATP酶活性、促进小鼠肠推进有关。Objective： To study the different purgative effects and molecular mechanisms among different components of processed Rhez Radix et Rhizoma （extracts from Rhez Radix et Rhizoma, cooked Rhez Radix et Rhizoma and Rhez Radix et Rhizoma carbon）. Method： Mice were randomly divided into control group and treatment groups with different components of processed Rhez Radix et Rhizoma at a dose of 10 g .kg-1. The different samples were administered to mice for observing the purgative number, the first purgative time, the 5 hour purgative times and defecation quantity. The effects on small intestinal propulsion were determined by propulsion rate of charcoal powder in mice. The effects on Na＋-K＋-ATP ase in rat colon wall cell membrane were determined after the rat was treated for 5 days. Result： The mice showed no purgative effect after be administered with different components of the processed Rhez Radix et Rhizoma except the component 2 and 3. As the total number of defecation in mice was concerned, except the cooked Rhez Radix et Rhizoma component 2 and 3 as well as the Rhez Radix et Rhizoma component 4 and Rhez Radix et Rhizoma carbon component 4, other components were significantly higher compared with the controls （P 〈 0.05 or P 〈 0.01 ）. Compared with the Rhez Radix et Rhizoma component 2, the number of defecation for the cooked Rhez Radix et Rhizoma component 2 was significantly less （P 〈 0.05）. Compared with the Rhez Radix et Rhizoma component 3, the number of defecation for the cooked Rhez Radix et Rhizoma component 3 and the Rhez Radix et Rhizoma carbon component 3 was significantly less （P 〈0.01）. Compared with control group, the Rhez Radix et Rhizoma component 2 and 3 could significantly promote the intestinal propulsion （P 〈 0. 05） , while the other components also had the same tendency. Among the different components of the same procesed Rhez Radix et Rhizoma, the intestinal propulsions of the Rhez Radix et Rhizoma component 2 and 3 were higher than the cooked Rhez Radix

关 键 词：大黄炮制品 组分 泻下作用 排便次数 小肠推进率 

分 类 号：R285.5[医药卫生—中药学]