复方丹参方与穿山龙总皂苷配伍对大鼠心肌缺血再灌注损伤的保护作用  

Effect of Fufang Danshenfang Combined with Total Saponin of Dioscorea on Myocardial Ischemia Reperfusion Injury in Rats

作　　者：潘从泽[1] 王云飞[1] 高秀梅[1] 王升启[2] 

机构地区：[1]天津中医药大学,天津300193 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《中国实验方剂学杂志》2012年第17期170-174,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金面上项目(81073093);国家科技部"重大新药创制"子课题(2011ZX09201-201)

摘　　要：目的:观察复方丹参方所含成分丹参提取物、三七总皂苷、冰片与穿山龙总皂苷配伍对大鼠心肌缺血再灌注损伤的保护作用。方法:SD大鼠70只,随机分为10组:假手术组、模型组、丹参提取物300 mg.kg-1、三七总皂苷100 mg.kg-1、穿山龙总皂苷100 mg.kg-1、配伍(丹参提取物+三七总皂苷+冰片+穿山龙总皂苷)3个剂量组(300+100+10+50),(300+100+10+100),(300+100+10+200)mg.kg-1、复方丹参片组300 mg.kg-1、阳性药组(通心络胶囊)300 mg.kg-1,各组ig给药,连续7 d,末次给药1 h后,结扎冠状动脉左前降支复制心肌缺血再灌注损伤模型,试剂盒测定血清超氧化物歧化酶(SOD)、乳酸脱氢酶(LDH)、肌酸激酶同工酶(CK-MB)活性,TTC染色法测定心肌梗死面积,TUNEL染色法检测细胞凋亡。结果:配伍(300+100+10+100)mg.kg-1与模型组比较,可显著增强SOD的活性(P<0.01),降低大鼠心肌中LDH和CK-MB的活性(P<0.01);与各成分和原方相比,心肌梗死范围为20.78%,明显低于丹参提取物34.04%(P<0.05)、三七总皂苷34.62%(P<0.05)、穿山龙总皂苷36.19%(P<0.05)、复方丹参片26.47%;细胞凋亡指数为16.53%,明显低于丹参提取物25.08%(P<0.05)、三七总皂苷22.12%(P<0.05)、穿山龙总皂苷24.45%(P<0.05)、复方丹参片18.14%。结论:复方丹参方与穿山龙总皂苷配伍后可对心肌缺血再灌注损伤起到保护作用,且效果优于各组分及复方丹参方。此种配伍的方式可以作为研究中药新复方的一种方法。Objective： To observe the protective effect of Fufang Danshenfang and total saponin of Dioscorea compatibility on myocardial ischemia reperfusion injury in rats. Method： Seventy SD rats were randomly divided into 10 groups： sham-operation group, model group, Salvia extract （300 mg·kg-1）, total saponins of panax notoginseng （PNS） 100 mg·kg-1, dioseorea saponins 100 mg ·kg-1, compatibility （Salvia extract ＋ PNS ＋ borneol ＋ Dioscorea saponins） three dose of （300 ＋ 100 ＋ 10 ＋50） , （300 ＋ 100 ＋ 10 ＋ 100） , （300 ＋ 100 ＋ 10 ＋ 200） mg·kg-1, compound Danshen tablets 300 mg·kg-1 , Tongxinluo capsules 300mg·kg-1. Eaeh group was ig administrted for 7 consecutive days. The model of myocardial ischemia reperfusion injury in rats was induced by ligation of the left anterior descending of the coronary artery （LAD） after one hour of end administration. Serumsuperoxide dismutase （SOD） , lactic clehydrogense （LDH） and creatine kinase isoenzyme （CK-MB） activity were determined by kit, the TTC staining method was used to determine the myocardial infarct size, TUNEL staining was used to detect apoptosis. Result： Compatibility （300 ＋ 100 ＋ 10 ＋ 100） mg·kg-1 could significantly enhance SOD activity （P 〈 0.01 ） significantly reduce myocardial LDH and CK-MB activity （P 〈 0. 01 ）; compared with the ingredients and the original side, infarct size of 20.78% was significantly lower than the Salvia extract 34.04% （P 〈 0. 05）, PNS 34.62% （P 〈 0.05 ）, the total saponin of Dioscorea 36. 19% （P 〈 0.05 ）, Fufang Danshen Tablets 26.47% ; apoptosis rate of 16.53% , 25.08% （P 〈 0. 05 ） was significantly lower than the Salvia extract 25.08% , PNS 22. 12% （ P 〈0. 05 ）, the total saponin of Dioscorea 24.45% （ P 〈 0.05 ）, Fufang Danshen Tablets 18. 14%. Conclusion： Fufang Danshenfang and the total saponin of Dioscorea compatibility have showed protective effects on myocardial ischemia reperfusion injury, which is much

关 键 词：复方丹参方 穿山龙总皂苷 心肌缺血再灌注 缺血面积 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]