竹节参总皂苷对H_2O_2致乳鼠心肌细胞氧化应激损伤的保护作用  

Protective Effects of Total Saponin from Panax japonicus on H_2O_2 Induced Oxidative Stress in Neonatal Rat Cardiomyocytes

作　　者：贺海波[1,2] 徐媛青[1] 魏娜[1] 代艳文[1] 关乔中[1] 张长城[1] 王婷[1] 袁丁[1] 

机构地区：[1]三峡大学医学院,湖北宜昌443002 [2]三峡大学天然产物研究与利用湖北省重点实验室,湖北宜昌443002

出　　处：《中国实验方剂学杂志》2012年第17期187-191,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(31070314;30873383)

摘　　要：目的:通过建立H2O2诱导心肌细胞氧化应激损伤模型,观察竹节参总皂苷(SPJ)对心肌细胞的保护作用。方法:将原代培养的乳鼠心肌细胞随机分为正常组,模型组(H2O2),SPJ低剂量组(50 mg.L-1),SPJ高剂量组(100 mg.L-1)。H2O2诱导心肌细胞氧化损伤2 h后SPJ孵育24 h,显微镜下观察细胞搏动频率,MTT法测定细胞存活率,比色法测定细胞培养上清液中乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)的活性及丙二醛(MDA)含量。结果:SPJ(50,100 mg.L-1)可明显增加心肌细胞搏动频率,降低H2O2所致乳鼠心肌细胞LDH释放量,升高SOD,CAT,GSH-Px活性,降低MDA含量(P<0.01或P<0.05)。结论:SPJ对H2O2所致乳鼠心肌细胞氧化应激损伤有明显保护作用。Objective： Through the establishment of the model of oxidatie stress damage induced by H2O2in myocardium cells, to observe the protective effects of total saponin from Panax japonicus （SPJ） on myocardial cells. Method： Neonatal rat cardiomyocytes were randomly divided into four groups： normal, model （H202）,H2O2 ＋I-SPJ （SPJ, 50 mg · L-1） and H2O2 ＋ h-SPJ （SPJ, 100 mg· L-l）. H2O2was used to induce oxidative stress injury in primary cultured cardiomyocytes of neonatal rats for 2 hours, and then incubated 24 hours with SPJ （50, 100 mg· L-l）. The beating rates of cardiomyocytes were monitored under inverted microscope, cell viability was detected by MTT assay. The content of lactate dehydrogenase （LDH） , malondialdehyde （MDA） , and the activities of superoxide dismutase （SOD） , catalase （CAT） , glutathione peroxidase （GSH-Px） in culture medium were detected after treated by SPJ with colorimetric technique at 24 hours. Result： SPJ could significantly increase beating rates of cardiomyocytes; and the contents of LDH, MDA in culture medium were remarkably decreased, and activities of SOD, CAT and GSH-Px were significantly increased （P 〈 0.05 or P〈 0.01 ）. Conclusion： SPJ exerted protective effects on oxidative stress injury induced by exerted protective effects on oxidative stress injury induced by H2O2 in eardiomyocytes of neonatal rats.

关 键 词：竹节参总皂苷 H2O2 心肌细胞 氧化应激 抗氧化作用 

分 类 号：R283.6[医药卫生—中药学]