CdTe量子点荧光猝灭法测定奥沙利铂中微量银  

Determination of trace amounts of silver in oxaliplatin by fluorescence quenching of CdTe quantum dots

作　　者：董微[1] 徐淑坤[2] 王莹[3] 

机构地区：[1]沈阳医学院化学教研室,沈阳110034 [2]东北大学化学系,沈阳110819 [3]辽宁大学生命科学院,沈阳110036

出　　处：《分析试验室》2012年第9期5-8,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然基金资助项目(20875011);辽宁省自然基金资助项目(20102221);辽宁省教育厅资助项目(L2010542);沈阳市科技局资助项目(F11-262-9-21)资助

摘　　要：以谷胱甘肽作为稳定剂,100℃恒温回流,直接合成水溶性CdTe量子点。基于Ag+对合成的CdTe量子点的荧光猝灭效应,建立了测定抗癌药物奥沙利铂中微量银的方法。考察了量子点浓度、缓冲液种类、缓冲液浓度、缓冲液pH和反应时间对银离子测定的影响。当量子点浓度为0.004 g/L时,在0.10 mmol/LpH7.4的磷酸缓冲溶液中,反应时间为5 min,体系的相对荧光强度与Ag+的质量浓度呈良好的线性关系,其线性范围为16.42～98.50μg/L,线性相关系数为0.9975,检出限为0.12μg/L。Water-soluble CdTe quantum dots （QDs） were synthesized by means of L-glutathione （GSH） as a modifying agent in an aqueous solution at 100 C. Based on the quenching of the fluorescence intensity of CdTe QDs by Ag（I） , a simple, rapid and sensitive method for the determination of trace amounts of silver in anti- cancer drug oxaliplatin was developed. In the study, the effects of experimental conditions, including concentration of CdTe QDs, the type, concentration and pH of buffer, and reacting time on the determination of Ag （I） were investigated in detail. When the concentration of QDs was 0. 004 g/L in the phosphate buffer at pH 7.4, the fluorescence quenching intensity of the CdTe QDs was linear with Ag （I） mass concentration in the range of 16.42 - 98.50 μg/L, with a linear correlation coefficient of 0. 9975. The limit of detection （3 （ ） was O. 12 μg/L.

关 键 词：量子点 荧光猝灭 奥沙利铂 银 

分 类 号：O657.3[理学—分析化学]