一例慢性移植物抗宿主病患者TCRVβT细胞分布和克隆性的研究  

Evidence for TCR Vβ clonal expansion T cells in a patient with cGVHD after unrelated donor BMT

作　　者：杜欣[1] 李扬秋[2] 翁建宇[1] 陆泽生[1] 苏建华[1] 林伟[1] 黄梓伦[1] 

机构地区：[1]广东省人民医院血液科,广州510080 [2]暨南大学血液研究室

出　　处：《中华血液学杂志》2000年第8期406-408,共3页Chinese Journal of Hematology

基　　金：广东省卫生厅医学科研基金!资助项目 (A1998)

摘　　要：目的　了解非血缘关系骨髓移植后慢性移植物抗宿主病 (cGVHD)患者的T细胞受体(TCR)VβT细胞的分布和克隆性。方法　应用逆转录 聚合酶链反应 (RT PCR)扩增 1例非血缘关系骨髓移植后cGVHD患者的外周血单个核细胞的TCRVβ 2 4个亚家族的CDR3,了解患者TCRVβT细胞的分布情况 ,PCR产物进一步经基因扫描分析确定T细胞克隆性。结果　患者存在 8个TCRVβ亚家族T细胞 ;其中Vβ2、Vβ3、Vβ8和Vβ13亚家族为克隆性增殖T细胞。 结论　患者外周血出现不均匀性 (即倾斜性 )分布和克隆性生长TCRVβ家族T细胞 ,它可能与cGVHD的发生有关。Objective To investigate the distribution and clonal expansion of TCR Vβ subfamily T cells in patients with cGVHD after unrelated donor bone marrow transplantation (BMT). Methods The CDR3 of 24 TCR Vβ subfamily genes were amplified from peripheral mononuclear cells of a patient with cGVHD after unrelated BMT. RT PCR was used for detection of the distribution of TCR Vβ repertoire, and the PCR products were further analyzed by genescan technique for the CDR3 size, to evaluating clonality of the detectable TCR VβT cells. Results Only 8 Vβ subfamily T cells could be identified in the patient, clonal expansion T cells could be found of Vβ2, 3, 8 and 13 subfamilies. Conclusions The skew distribution and clonal expansion of TCR Vβ subfamily T cells could be found in the patient. It may relate to the initiation of cGVHD.

关 键 词：T细胞受体VΒ 骨髓移植 移植物抗宿主病 克隆 

分 类 号：R392[医药卫生—免疫学]