利用ITS基因研究徐闻滨珊瑚的系统发生关系  

Phylogeny analysis of Porites link in Xuwen using its gene

作　　者：李文娟[1] 刘楚吾[1] 刘丽[1] 廖宝林 

机构地区：[1]广东海洋大学水产学院,南海水产经济动物增养殖广东省普通高校重点实验室,广东湛江524025 [2]徐闻珊瑚礁国家级自然保护区,广东湛江524100

出　　处：《海洋科学》2012年第7期56-60,共5页Marine Sciences

基　　金：广东省海洋渔业科技推广专项(A200908F01);国家海洋公益性行业科研专项(201105012)

摘　　要：分别研究了徐闻3种滨珊瑚的ITS1和ITS2基因的碱基组成和G+C含量,并和已上传至Genbank上的其他9种滨珊瑚的ITS序列进行比较,研究了徐闻3种滨珊瑚的系统发生关系。序列分析结果显示,3种滨珊瑚的ITS1的长度为205 bp～209 bp,G+C含量为37.6%～45.5%,ITS2的长度为192 bp～226 bp,G+C含量为45.1%～50.7%,利用MEGA4.1软件计算滨珊瑚属基于ITS1和ITS2基因的平均遗传距离分别为0.097和0.200。基于ITS1和ITS2的NJ系统进化树都显示出,灰黑滨珊瑚位于进化树的基部,是原始的类群,普格滨珊瑚是进化类群,澄黄滨珊瑚是过渡类群。The obtained ITS （internal transcribed spacer） sequences of 3 species in Porites by PCR technique were analyzed by MEGA4.1 software to get base composition, G＋C content and phylogenetic relationship with ITS gene of other 9 species uploaded to Genbank, The results showed that the ranges of ITS1 and ITS2 of the tested 3 species were 205-209 bp and 197-226 bp, and the ranges of G＋C content of ITS1 and ITS2 were 37.6%-45.5% and 45.1%-50.7%. The average genetic distance based on ITS1 and ITS2 were 0.097 and 0.200 respectively .The N J-based phylogenetic tree based both ITS 1 and ITS2 shows that Porites nigrescens at the base of evolutionary tree, is a primitive group, Porites pukoensis is an evolutionary group and Porites lutea is a transitional group.

关 键 词：滨珊瑚 ITS基因 系统发生关系 

分 类 号：Q959[生物学—动物学]