血必净对大鼠急性肺损伤后肺纤维化的干预效应研究  

Effect of Xuebijing on lung fibrosis in a rat model of oleic acid-induced lung injury

作　　者：梁子敬[1] 汪友平[1] 赖荣德[1] 

机构地区：[1]广州医学院第一附属医院急诊科,广州510120

出　　处：《中华急诊医学杂志》2012年第8期840-845,共6页Chinese Journal of Emergency Medicine

基　　金：广州市医药卫生科技项目（2009-YB-237）

摘　　要：目的探讨血必净对油酸诱导大鼠急性肺损伤后肺纤维化的影响。方法雄性SPF级Wistar大鼠60只，随机（随机数字法）分为3组（n=20）：A组注射生理盐水作为健康对照组；B、C两组所有大鼠均用油酸复制急性肺损伤模型，B组为模型对照组，C组在模型基础上加用血必净为干预组。3组均于注射后12h抽动脉血作血气分析测定血氧分压，以氧合指数≤300为造模成功。确定模型成功后，A、B两组经尾静脉注射生理盐水（4ml／kg），C组经尾静脉给予血必净（4ml／kg），每天1次，连续给药7d。各组于给药后24h、7d、14d和28d各处死5只大鼠，取心脏血后处死大鼠，左肺称湿质量后行支气管肺泡灌洗，灌洗后烘干称质量，酶联免疫法（ELISA）检测血清和支气管肺泡灌洗液Ⅲ前胶原（PCⅢ）浓度，取右肺上叶行肺组织病理学检查，经石蜡包埋切片后分别行苏木素一伊红（HE）染色和Masson染色。各组间比较采用单因素方差分析，均数间差别多重比较采用SNK-q检验；非正态分布或方差不齐采用秩和检验。所有数据均用SPSS13．0统计软件包进行统计处理。结果B、C两组氧合指数（Pa02／Fi02）〈300，与A组比较明显下降（P〈0．05）；与A组相比，B、C组在7、14、28d3个时点血清和支气管肺泡灌洗液中Ⅲ前胶原蛋白的浓度增高（P〈0．01），C组与B组相比在14d和28d两时点血清PCⅢ浓度降低（P〈0．05），C组在7、1d和28d时点支气管肺泡灌洗中Ⅲ前胶原蛋白的浓度比同时段的B组降低（P〈0．01）。结论血必净可能通过抑制急性肺损伤后肺纤维化而有助于损伤肺保护。Objective To investigate the effect of Xuebijing （Chinese herbal medicine preparation） on lung fibrosis in a rat model of oleic acid - induced lung injury. Methods Sixty SPF level of male Wistar rats were randomly （random number） divided into three groups （n = 20）： normal control group （group A）, oleic acid model group （group B）, oleic acid and Xuebijing group （group C）. Rats in group A were injected with normal saline （0. 13 ml/kg） ; acute lung injury （ALI） model rats were injected with oleic acid （0. 13 ml/kg） intravenously in B and C groups. Twelve hours after administrations of saline or oleic acid into rats of three groups separately, samples of arterial blood from all rats were taken for blood gas analysis. By analysis of blood oxygen partial pressure, the acute lung injury model successfully made was confirmed. Then the rats in group C were treated with xuebijing （4 ml/kg） intravenously, once a day from the 1st to 7th day. The rats in groups A and B were treated with saline （4 ml/kg）. Five rats in each group were randomly sacrificed on the 1 st , 7th, 14th, 28th day, separately. The bronchoalveolar iavage （BAL） of left ~ung of all rats was carried out after meig^t of lung meigbed. The left lungs of rats were weighed in wet and dry in order to get the wet/dry ratio. Venous blood and BAL fluid （BALF） were obtained for detection of procollagen Ill （ PC m ） concentrations with enzyme - linked immunosorbent assay （EL1SA）. Right upper lobe of lung specimens was taken for histopathological examination after paraffin - embedded sections with hematoxylin-eosin （HE） staining and Masson staining. Multiple comparisons were analyzed by using one- way analysis of variance （SNK-q test）. Statistical analyses were performed by using SPSS 13.0 software. Results Oxygenation index of groups B and C （PaOJFiO2 〈 300） significantly decreased compared with the group A （ P 〈 0. 01 ）. Levels of serum PC lU on the 14th day and 28th day in group C decre

关 键 词：急性肺损伤 急性呼吸窘迫综合征 肺纤维化 血必净注射液 油酸 11I前胶原蛋白 HE染色 MASSON染色 

分 类 号：R563.8[医药卫生—呼吸系统]