高压氧对大鼠T淋巴细胞和细胞因子的影响  

Effect of hyperbaric oxygen on T.lymphoid ceils and cytokines in rats

作　　者：刘志红[1] 冯芳[2] 王钢[1] 

机构地区：[1]中国医科大学附属第一医院急诊科,沈阳110001 [2]沈阳市苏家屯区中心医院

出　　处：《中华急诊医学杂志》2012年第8期846-850,共5页Chinese Journal of Emergency Medicine

摘　　要：目的研究高压氧对健康大鼠以及全身炎症反应综合征（SIRS）大鼠T淋巴细胞和细胞因子（IL-6、IL一10、TNF-d）等的影响。方法40只雄性SD大鼠随机（随机数字法）分为5组。A组：腹腔注射生理盐水（0．9％NaCl，5ml／kg）作为对照组；B组：腹腔注射等量生理盐水，做3次高压氧治疗；C组：腹腔注射酵母多糖一石蜡悬液（500mg／kg）；D组：腹腔注射等量酵母多糖一石蜡悬液，做1次高压氧治疗；E组：腹腔注射等量酵母多糖一石蜡悬液，做3次高压氧治疗。所有大鼠均于腹腔注射24h后处死，门静脉取血，标本采用流式细胞技术对CD3’CD4’CD8‘进行计数，应用ELISA方法检测IL-6、IL-10、TNF—o【血浆水平，以单因素方差分析及LSD—t检验分析数据。结果高压氧治疗可显著降低健康大鼠外周血CIMT细胞百分比（P〈0．01），使CIM／CD8T细胞比值下降（P〈0．05）。酵母多糖腹腔注射使大鼠外周血CIM、CD8T细胞均减少（P〈0．01）。1次和3次HBO治疗可使酵母多糖所致CD4T细胞减少明显恢复（P〈0．01，P〈0．05）。对于未出现高炎症反应的大鼠，HBO治疗可增加IL-6（P〈0．05）和TNF—d（P〈0．01），而减少IL一10（P〈0．05）。结论高压氧可降低健康大鼠外周血CD4T细胞百分比。当出现高炎症反应时，高压氧可以明显抑制细胞因子TNF一仅、IL-6和IL一10的爆发增多。高压氧对健康大鼠和未出现高炎症反应的SIRS大鼠起促炎作用。Objective To investigate the effects of hyperbaric oxygen therapy on T-lymphocytes I and eytokines including IL-6, IL-IO and TNF-c~ as indicators well representing immune function and inflammation in normal rats and rats with systemic inflammatory response syndrome. Methods Forty Sprague-Dawley male Rats were randomly （ random number） divided into five groups. Rats of group A were treated by saline solution （0. 9 % NaC1, 5 inl/kg）, and served as control group; rats of group B were treated with saline solution and hyperbaric oxygen therapy three times; rats of group C were treated with zymosan （500 mg,/kg） ; rats of group D were treated with zymosan and hyperbaric oxygen therapy once; and rats of group E were treated with zymosan and hyperbaric oxygen therapy three times. At 24 hours after saline or zymosan administration, rats were sacrificed and blood samples were collected from the portal vein. CD3 ~ , CIM ＋ , CD8 ＋ ceils were counted by flow cytometer, and plasma levels of IL-6, IL-10 and TNF-c~ were measured by ELISA . The results were analyzed by one - way ANOVA and LSD post-hoe test. Results CD4 T cell of normal rats was decreased by hyperbaric oxygen （ P 〈 0. 01 ） , CD4/CD8 T cell ratio was also decreased （P 〈0.05）. Of rats with zymosan administration, CIM T cells and CD8 T cells were both decreased on a parallel （ P 〈 O. 01 ）. A single one HBO and HBO for three times both raised the percentage of CIM T cells which was significantly decreased by zymosan administration （ P 〈 0. O1 and P 〈 0. 05 ）. In rats without hyper- inflammation, hyperbaric oxygen therapy showed a pro-inflammatory effect by elevating TNF-ct （P〈0.01）, IL-6 （P〈0.05） and reducing IL-10 （P〈0.05）. Conclusions HBO reduces CI）4 T cells in normal rats. HBO reduces the production of TNF-a, IL-6 and IL-10 during hyper - inflammation. HBO is pro - inflammatory in normal rats and minor SIRS rats withourt appreciable hyper-inflammation in circulation.

关 键 词：高压氧 SIRS T淋巴细胞 细胞因子 免疫功能 TNF-A 

分 类 号：R459.7[医药卫生—急诊医学]