天津地区人群中LOX-1基因3＇-UTR多态性与冠心病的关系  

Correlation between Polymorphism of 3'-UTR of Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 Gene and Coronary Artery Disease in Tianjin Population

作　　者：豆静[1] 刘寅[1] 高静[2] 毛用敏[2] 张倩 蔡泽园[2] 崔让庄[2] 王林[1] 孙根义[1] 

机构地区：[1]天津市胸科医院心内科,300051 [2]天津市胸科医院心血管病研究所,300051

出　　处：《天津医药》2012年第9期885-888,共4页Tianjin Medical Journal

摘　　要：目的:研究天津地区人群中凝集素样氧化低密度脂蛋白受体-1(LOX-1)3′-非编码区(3′-UTR)188位点基因多态性情况,探讨LOX-1基因多态性与冠心病(CAD)及血脂水平的关系。方法:冠心病组170例,均经冠脉造影(CAG)确诊;对照组140例,均经CAG排查。应用聚合酶链反应-限制片段长度多态性(PCR-RFLP)法对研究对象的LOX-1基因3′-UTR188位点多态性进行分析,并用自动生化分析仪检测血浆血脂水平。结果:冠心病组和对照组比较,LOX-1基因3′-UTR 188位点基因型频率和等位基因频率差异均无统计学意义(均P>0.05);在总研究对象中T等位基因携带者(CT+TT)与CC基因型者血三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、脂蛋白(a)[Lp(a)]水平差异均无统计学意义(均P>0.05),冠心病组与对照组中的CT+TT基因型者以上血脂水平差异亦无统计学意义(P>0.05)。结论:天津地区人群中LOX-1基因3′-UTR188位点多态性与冠心病的发病及血脂水平均无明显关系,其不能作为冠心病的预测因素。Abstract Objective： To investigate the polymorphism of 3＇-UTR 188 site of lectin-like oxidized low density lipoprotein receptor-1 （LOX-I） gene and to explore the correlation between the gene polymorphism of LOX-1 and coronary artery disease （CAD）and lipid levels in the population of Tianjin. Methods： A total of 170 cases confirmed by coronary arteriograghy （CAG） were included in CAD group and 140 healthy subjects confirmed by CAG were included in control group. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method was used to analyze the polymorphism of 3＇-UTR 188 site of LOX-1 gene and to compare its relationship with coronary artery disease between the two groups. The automatic biochemistry analyzer was used to detect plasma lipid levels of all subjects. Results： There was no significant difference in genotype frequency and allele frequency of 3＇-UTR 188 site of LOX-1 between CAD group and control group （P 〉 0.05）. There were no significant differences in triglyceride （TG）, total cholesterol （TC）, low density lipoprotein cholesterol （LDL-C）, high density lipoprotein cholesterol （HDL-C） and lipoprotein （a） levels between T allele （CT＋TI＇） and CC genotype （P 〉 0.05）. There was no significant difference in lipid levels between CAD group and control group of subjects with CC＋TT genotype. Conclusion： There is no significant correlation between the polymorphism of 3＇- UTR 188 site of LOX-1, coronary artery disease and lipid levels in the population of Tianjin, therefore the polymorphism can not be used as a predictor of coronary artery disease.

关 键 词：凝集素类 受体 氧化LDL冠状动脉疾病 冠状血管造影术 等位基因 多态性 限制性片段长度 凝集素样氧化低密度脂蛋白受体-1 

分 类 号：R541.4[医药卫生—心血管疾病]