高效液相色谱法测定甲鱼油中EPA、DHA的含量  

High Performance Liquid Chromatographic Determination of DHA and EPA in Turtle Oil

作　　者：赖春华[1,2] 李军生[1] 廖永聪 廖诚珍 谢志扬 陈铁英 

机构地区：[1]广西工学院生物与化学工程系,广西柳州545006 [2]柳州市产品质量监督检验所,广西柳州545006 [3]贵港市水产技术推广站,广西贵港537100

出　　处：《食品研究与开发》2012年第7期102-104,共3页Food Research and Development

基　　金：广西贵港科学研究与技术开发计划项目(贵科软0906013)

摘　　要：建立分离检测广西黄沙鳖甲鱼油中的EPA和DHA的高效液相色谱法。试验采用岛津VP-ODS色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇和水(体积比70∶30),紫外检测波长为254 nm,流速为0.7 mL/min,柱温为30℃。甲鱼油经0.5 mol/L KOH-乙醇溶液进行皂化,三乙胺-溴苯乙酮酯化后直接上机分析。结果表明:EPA、DHA在0.025 mg/mL^0.15 mg/mL范围内线性关系良好,R2EPA为0.999 1、R2DHA为0.999 5;该法用于甲鱼油的检测,回收率EPA为96.00%～98.67%,DHA为92.00%～98.00%,该法简便、快速、重现性好。A high performance liquid chromatographic （HPLC） method was established for the determination of EPA and DHA in Chinese yellow sand soft-shelled turtle oil.The analysis was performed on shimADZU-PACK VP-ODS colunm （250 mm × 4.6 mm, 5 um） at the detection wave length of 254 nm. The mobile phase was consisted of methanol- water （volume ratio was 70：30）as a flow rate of 0.7 mL/min at column temperature 30 ℃. The tutrle oil saponificatied by 0.5 mol/L KOH-ethanol solution and estefified by Triethylamine-a- bromoacetophenone after analysis. The results showed that the calibration curve was linear in the concentration range of 0.025 mg/mL -0.15 mg/mL （R2 FPA =0.999 1, R2 DRA=0.999 5）. The recoveries ranged from 96.00 % to 98.67 % for EPA and 92.00 %-98.00 % for DHA. The method was fast ,simple and accurate.

关 键 词：黄沙鳖 DHA和EPA 高效液相色谱 甲鱼油 

分 类 号：TS227[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.72[轻工技术与工程—食品科学与工程]