反相高效液相色谱法测定氨茶碱的血药浓度  

HPLC Determination of Aminophylline Concentration in Human Serum

作　　者：苏煌财[1] 林玉仙[1] 张宏[1] 

机构地区：[1]福建医科大学附属福州市第一医院临床药学科,福建福州350009

出　　处：《药品评价》2012年第8期25-27,共3页Drug Evaluation

摘　　要：目的:探讨了高效液相色谱法测定人血清中氨茶碱的药物浓度的方法。方法:采用symmetryshield RP18柱(5.0μm,3.9×150 mm)为色谱柱。流动相为甲醇∶水为15:85,流速1.0mL.min-1;Waters 2487双波长紫外检测器,检测波长272nm;乙酰苯胺为内标物。结果:线性范围为(4.4～33.0)μg.mL-1,r=0.999190,最低检测限0.16μg.mL-1;平均回收率为96.7%,高、中、低浓度样品的日间和日内测定相对标准偏差RSD均小于2%。结论:本法适用于氨茶碱的血药浓度测定。Objective： To establish a HPLC method for determining human serum level of aminophylline. Methods： Acetanilide was selected as the internal standard. A symmetryshield RP18 column（5μm,3.9×150mm）was used. The mobile phase, methanol-phosphate solution（15：85）was used at a flow rate 1.0 mL·min^-1. The detector is Waters 2487 Dual λ Absorbance Detector, and was set at the wavelength of 272 nm. Results： The calibration curve was linear in the range of 0.16 to 33.0μg·mL^-1. The average recovery was 96.7%; the RSD within-day and between-day were all less than 2%. Conclusion： This method is suitable for therapeutic drug monitoring of aminophylline.

关 键 词：高效液相色谱法 氨茶碱 血药浓度 

分 类 号：R969.4[医药卫生—药理学]