两株红色糖多孢菌在不同氮源培养条件下的代谢比较  

Metabolism Comparison of Two Strains of S.erythraea in Different Nitrogen Sources

作　　者：黄刚[1] 周英[1] 叶邦策[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《化学与生物工程》2012年第8期59-62,共4页Chemistry & Bioengineering

摘　　要：转录组芯片和凝胶阻滞(EMSA)等实验发现,在红霉素高产菌株中,与氮源代谢相关的基因(如glnR、gl-nB)、铵盐与寡肽转运蛋白基因等在高产菌株中被强烈抑制,可推测在高产菌株和野生菌株中,氮源代谢存在很大差异,并与红霉素合成存在紧密的联系。通过测定4种单一氮源培养条件下的野生模式菌(M菌)和高产菌(A菌)体内的有机酸和氨基酸含量来比较其代谢情况。结果表明,在同一菌株内及不同菌株间,与氮源代谢直接相关的α-酮戊二酸、谷氨酸、谷氨酰胺的含量及与碳源代谢相关的有机酸如苹果酸、柠檬酸的含量在不同的培养基内呈现相反的趋势。Through detecting the contents of amino acids and organic acids in the cells of two strains of S.erythraea including an industrial strain（strain A） and a wide-type strain（strain M） in four kinds of single nitrogen source,their nitrogen metabolism were compared.The results showed that,whether in same strain or in different strains,the contents of α-ketoglutaric acid,glutamic acid,glutamine belonged to the nitrogen metabolism system had the opposite trend with those of citric acid,malic acid belonged to the carbon metabolism system in different media.

关 键 词：红色糖多孢菌 氮源 Α-酮戊二酸 代谢 

分 类 号：TQ465.5[化学工程—制药化工]