超高效液相色谱-串联质谱法测定化妆品中苯菌灵和多菌灵  

Determination of benomyl and carbendazim in cosmetics by ultra performance liquid chromatography-tandem mass spectrometry

作　　者：薛霞[1,2,3] 宿书芳[1,2,3] 王骏[1,2,3] 张卉[1,2,3] 祝建华[1,2,3] 

机构地区：[1]山东省产品质量监督检验研究院 [2]国家加工食品质量监督检验中心(山东) [3]山东省材料化学安全检测技术重点实验室,济南250103

出　　处：《中国卫生检验杂志》2012年第8期1796-1799,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立超高效液相色谱-串联质谱(UPLC-MS/MS)法测定化妆品中微量苯菌灵和多菌灵的方法。方法:试样用甲醇-盐酸水溶液提取后,经正己烷萃取除去脂溶性杂质,以混合机理的阳离子固相萃取柱净化、富集,最后C18色谱柱分离,采用UPLC-MS/MS电喷雾电离(ESI),正离子,多反应监测(MRM)模式检测,外标法定量。结果:在1 ng/ml～100 ng/ml范围内,线性关系良好,r2优于0.99;添加回收率范围为82.0%～95.6%,相对标准偏差(RSD)小于4.8%,定量下限为1.0μg/kg。结论:该方法适合各类化妆品中苯菌灵和多菌灵的测定。Objective： To establish an ultra performance liquid chromatographytandem mass spectrometric method for determination of benomyl and carbendazim in cosmetics. Methods： The samples were extracted with methanol aqueous solution of hydrochloric acid, the fat - soluble impurities were extracted with hexane, and then purified and enriched with MCX solid phase extraction. The sample solution was injected into UPLC - MS/MS. The analysis was carried out in the multiple reaction monitoring （MRM） mode by employing the external standard method. Results： Within 1 ng/ml- 100 ng/ml, the method showed good linearity （r^2 〉0.99）, The average recoveries were between 82.0% -95.6% with RSD less than 4.8%. The limit of quantitation （LOQ） was 1.0 μg/kg. Conclution： The method could be used as a reliable means for quantitative determination of benomyl and carbendazim in cosmetics.

关 键 词：超高效液相色谱-串联质谱 苯菌灵 多菌灵 化妆品 多反应监测 

分 类 号：O657.63[理学—分析化学]