基于M基因构建的麻疹病毒实时荧光PCR检测技术  

Study on Real-time PCR in detection of measles virus based on M gene

作　　者：杨贵清[1] 卓菲[1] 刘卫民[1] 何梅英[1] 文凤兰[1] 

机构地区：[1]广东省深圳市罗湖区疾病预防控制中心,广东深圳518020

出　　处：《中国卫生检验杂志》2012年第8期1843-1844,1847,共3页Chinese Journal of Health Laboratory Technology

基　　金：深圳市科技计划项目(医疗卫生类)(200803160)

摘　　要：目的:建立麻疹病毒的实时荧光PCR检测方法并应用于临床标本的检测。方法:根据四十年来在我国流行的麻疹病毒M基因序列,设计用于病毒实时荧光PCR检测的引物和探针,建立麻疹病毒实时荧光PCR检测方法。用此法对40例麻疹的病人标本进行检测,并与血清学检测结果比较。结果:通过40例病人标本中实时荧光PCR检测,39份阳性,1份阴性,阳性率97.5%,高于血清学检测(阳性率77.5%)。结论:该方法方便快捷、特异性强、敏感性高,可用于麻疹病毒的快速诊断和分子流行病学调查。Objective：To establish Real -time PCR method for detection of measles virus in clinic. Methods： According to M gene sequences of measles virus found in China in recent 40 years, the Real - Time PCR primers and probes were designed to establish Real - time PCR for detection of measles virus. Then specimens from 40 measles patients were detected by Real - time PCR and the resuh was compared with that of serological detection. Results： 40 samples of clinical patients were detected by Real - Time PCR. The results showed that 39 samples were positive and only 1 was negative. The positive rate was 97.5 % , to be much higher than that of serological detection method （77.5 % ）. Conclusion： The Real - Time PCR detection method was more convenient, specific and sensi- tive, which can provide technical support for rapid diagnosis of measles virus and investigation of molecular epidemiology.

关 键 词：麻疹病毒 实时荧光PCR M基因 

分 类 号：R511.1[医药卫生—内科学]