CMIA法测定梅毒螺旋体抗体在临床筛检梅毒的应用  

Evaluation of a chemiluminescent microparticle immunoassay for determination of Treponema pallidum antibodies in syphilis screening test

作　　者：金国江[1] 邹大伟[1] 杜毅鑫[1] 陈俊丽[1] 

机构地区：[1]中国医科大学附属第一医院检验科,沈阳110001

出　　处：《中国卫生检验杂志》2012年第8期1890-1891,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:探讨CMIA法测定梅毒螺旋体抗体在临床筛检梅毒的应用。方法:用CMIA法测定患者梅毒螺旋体抗体,阳性标本再进行TPPA确认试验,用ROC曲线分析确定S/CO值筛检梅毒精确度。结果:在28468例受检患者中,CMIA法梅毒抗体阳性501例,其中TPPA阳性413例,通过ROC曲线分析确定S/CO为3.2时(曲线下面积0.953,95%可信区间0.936～0.970),预测梅毒精确度为(敏感性88.8%,特异性94.4%)。结论:CMIA法检测梅毒抗体可以准确预测梅毒感染,根据S/CO值可以确定是否需要进行TPPA确认试验。Objective： To explore the clinical application of a chemiluminescent microparticle immunoassay （CMIA） for determination of Treponema pallidum antibodies in syphilis screening test. Methods： All serum samples were detected for the Treponema pallidum antibodies by CMIA. Then the positive samples were confirmed by Treponema pallidum particle agglutination （TPPA）. Receiver - operating characteristic （ROC） curve analysis was performed to evaluate the diagnostic accuracy of Treponema pallidum antibody S/CO for syphilis diagnosis. Results： In total of 28,468 patients, 501 patients were CMIA positive, and 413 cases were TPPA positive. By ROC curve a- nalysis, Treponema pallidum antibody S/CO ratio （ area, O. 953 ; 95% confidence interval, O. 936 to O. 970） accurately predicted the presence of Syphilis, with a cutoff value of 3.2 （sensitivity, 88.8% ; specificity, 94.4% ）. Conclusion： The CMIA for determination of Treponema pallidum antibodies can predict syphilis infection accurately. The S/CO ratio can be used to determine whether a TPPA test should be performed.

关 键 词：梅毒螺旋体抗体 微粒子化学发光免疫测定 梅毒螺旋体颗粒凝集试验 

分 类 号：R446.6[医药卫生—诊断学]