不同来源的穿心莲药材HPLC指纹图谱研究  

Study on HPLC fingerprint of Andrographis paniculata(Burm.f.)Nees of different sources

作　　者：谢郁峰[1] 李斌[1] 陈卓瀚[1] 陈荣海[1] 郑海英[2] 郭丽冰[2] 

机构地区：[1]广东药学院药科学院,广东广州510006 [2]广东药学院中药学院,广东广州510006

出　　处：《广东药学院学报》2012年第4期410-414,共5页Academic Journal of Guangdong College of Pharmacy

基　　金：国家自然科学基金(81073045);广东药学院2010年学生创新实验项目

摘　　要：目的建立穿心莲药材的指纹图谱并进行聚类分析,为其科学评价及质量控制提供可靠的方法。方法采用Welch Ultimate XB-C18柱(250 mm×4.6 mm,5μm)色谱柱,以乙腈-水为流动相梯度洗脱,检测波长226 nm,流速1.0 mL·min-1,柱温25℃。采用中药色谱指纹图谱相似度评价系统(2004A版)对其进行了相似度计算,并利用SPSS统计分析软件进行了聚类分析。结果建立了穿心莲药材的指纹图谱,确定了10个共有峰为特征峰。相似度评价结果显示,规范化种植基地穿心莲药材相似度均大于0.91,70%以上的市售穿心莲药材相似度在0.86以上。根据系统聚类分析结果,将不同来源的12批穿心莲药材分为2类。结论该方法简便、准确、重复性好,可作为穿心莲药材质量评价的依据。Objective To establish an HPLC fingerprint for quality control of Andrographis paniculata（Burm.F.）Nees.by cluster analysis.Methods The chromatographic fingerprints were obtained by HPLC with Welch Ultimate XB-C18（250 mm ×4.6 mm,5 μm）.The mobile phase was acetonitrile-water solution with gradient elution.The detection wavelength was set at 226 nm.The flow rate was 1.0 mL· min^-1 and the column temperature was maintained at 25 ℃.The similarity assay of A.paniculata of different sources was carried out to evaluate their quality by Traditional Chinese Medicine fingerprint similarity evaluation system（2004A Edition）,and hierarchical cluster analysis was performed by SPSS software.Results Ten main characteristic peaks were selected in the standard fingerprint,and the similarity of samples from different GAP bases was over 0.91,and the similarity of 70% samples from different pharmacies was over 0.86.About 12 batches of A.paniculata from different sources were classified into two groups through the results of hierarchical cluster analysis.Conclusion The method with good reproducibility is simple and accurate,which can be used as a quality control method for A.paniculata.

关 键 词：穿心莲 HPLC 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]