Slit2-Robo1信号阻断抗体及对照抗体的制备、纯化和鉴定  

Purification and identification of monoclonal antibody against Slit2-Robo1 signaling

作　　者：亓翠玲[1] 郭四美[1] 叶杰[1] 韦平[1] 邱嘉丽[1] 王丽京[1] 

机构地区：[1]广东药学院基础学院,广东广州510006

出　　处：《广东药学院学报》2012年第4期442-444,共3页Academic Journal of Guangdong College of Pharmacy

基　　金：国家自然科学基金(30900764);广东省医学科研基金(A2011299)

摘　　要：目的纯化Slit2-Robo1信号阻断抗体(R5抗体)及对照抗体。方法给小鼠腹腔注射杂交瘤3B2.3和CRL-1608细胞,产生的腹水应用凝胶过滤层析法和Protein A亲和层析法纯化R5抗体及对照抗体,并用SDS-PAGE和BCA法检测纯化后单克隆抗体的纯度及浓度。结果单抗纯化后电泳重链和轻链都呈一条带,R5抗体及对照抗体的质量浓度分别为0.986 mg/mL和3.57 mg/mL。结论应用层析法和Protein A亲和层析法纯化抗体,能够得到纯度较高的单克隆抗体。Objective To purify and identify function-blocking monoclonal antibody（R5 antibody） against Slit2-Robo1 signaling.Methods 3B2.3 and CRL-1608 cells were injected into abdominal cavity of BALB/c mice.Affinity chromatography with protein A-Sepharose CL-4B was used to purify monoclonal antibody against Slit2-Robo1 signaling.The purified monoclonal antibody R5 was identified by SDS-PAGE and BCA.Results Purified monoclonal antibody R5 showed a single band by electrophoresis.The concentration of monoclonal antibody R5 and control antibody were 0.986 mg/mL and 3.57 mg/mL,respectively.Conclusion Affinity chromatography through protein A-Sepharose CL-4B can purify monoclonal antibody effectively.

关 键 词：Slit2-Robo1信号 单克隆抗体 亲和层析 纯化 

分 类 号：R392.11[医药卫生—免疫学]