鸡传染性法氏囊病超强毒LX株的分离鉴定  

Isolation and Identification of Very Virulent Infectious Bursal Disease Virus Strain LX

作　　者：刘爵[1] 刘尚高[2] 周蛟[1] 

机构地区：[1]北京市农林科学院畜牧兽医研究所,海淀100089 [2]中国农业大学动物医学院,北京海淀100094

出　　处：《中国兽医杂志》2000年第5期13-15,共3页Chinese Journal of Veterinary Medicine

摘　　要：从一免疫失败鸡场中分离到 1株鸡传染性法氏囊病野毒株 ,命名为 LX株。经血清学、鸡胚接种、病毒形态、外源病毒 (CAA、NDV和 IBV)排除试验等证实该分离物为纯净的 IBDV。人工感染试验表明 ,该L X株接种 8周龄 SPF鸡后第 2天鸡只精神沉郁 ,拉白色或绿色粪便 ,发病率为 10 0 % ,第 3～ 4天出现死亡高峰 ,而后很少引起死亡 ,死亡率达 92 .3% ,剖检可见全身性出血素质 ,法氏囊呈“紫葡萄样”外观 ,脾脏肿大 ,胸腺萎缩 ,骨髓脂肪化 ,肝脏稍见脂肪变等病变 ,表现了超强毒株的特点。接种鸡胚后 72～ 96 h引起鸡胚几乎全部死亡的规律性死亡特征。从而表明 ,该 L X分离株为 IBDV超强毒 。An infectious bursal disease virus(IBDV) field strain,designated strain LX,was isolated from a flock with immune failure.With the use of serological method,embryo inoculation test,electron microscopic observation ,and test for exotic viruses,the isolate was determined as a pure IBDV strain.Eight week old specific pathogen free(SPF)chickens inoculated with the strain developed severe clinical disease with a morbidity of 100%,and a death peak(up to 92.3% mortality)on the third or fourh day after inoculation.On pathological examinations,the most characteristic primary gross lesions were severe bursal haemorrhage and thymic atrophy,as well as the bone marrow with fatty change,accompanied by slight fatty liver and severe generalized hemorrhagic diathesis.Nigh day old chicken embryos were characterized by regular death with as high as 100% mortality 3 4 days after inoculation.These showed that the isolate LX was a very virulent IBDV strain.

关 键 词：鸡传染性法氏囊病 分离 鉴定 超强毒株 

分 类 号：S858.315.3[农业科学—临床兽医学]