丝素蛋白含量对纳米羟基磷灰石仿生矿化和体外细胞相容性的影响  

Effect of Silk Fibroin Content on the Bionic Mineralization and In Vitro Cellular Compatibility of Silk Fibroin/Hydroxyapaptite Nanocomposites

作　　者：朱云荣[1] 陈玉云[2] 许国华[1] 叶晓健[1] 钟健[2] 何丹农[2] 

机构地区：[1]长征医院,上海200003 [2]纳米技术及应用国家工程研究中心,上海200241

出　　处：《无机材料学报》2012年第8期883-886,共4页Journal of Inorganic Materials

基　　金：国家重点基础研究发展计划(2009CB930000);上海市自然科学基金(0852nm03100)~~

摘　　要：通过制备含有不同丝素蛋白(SF)含量的丝素蛋白/纳米羟基磷灰石复合材料,着重研究丝素蛋白含量对纳米羟基磷灰石(n-HA)仿生矿化过程中晶体成核与生长及体外细胞相容性的影响.结果表明:SF的加入对n-HA晶体的成核和生长具有明显的调控作用;丝素含量的大小对n-HA晶体成核和生长没有明显的区别,但对晶粒在有机大分子中的聚集状态有明显的影响:当SF含量不超过20wt%时,n-HA晶粒呈现放射状团簇,当SF含量超过20wt%时,n-HA晶粒无序团聚.体外细胞相容性结果显示,SF的加入可以促进材料与细胞的界面亲和性,但SF含量对这种亲和性的影响不明显,20wt%和30wt%的SF含量对增殖能力具有较强的促进作用.Silk fibroin and hydroxyapatite nanocomposites （SF/HA） with various SF content were prepared. Effect of Silk fibroin content on the bionic mineralization and cellular compatibility in vitro of HA nanocrystals （n-HA） was investigated. The results show that SF content has an obvious effect on the nucleation and growth of n-HA. However, though SF content does not show obvious difference on the nucleation and growth of n-HA, it has obvi- ous effect on the n-HA aggregation. When SF content is less than 20wt%, n-HA orderly disperses in SF matrix. While the SF content is more than 20wt%, the n-HA aggregation becomes disordered. The in vitro cellular compati- bility experiments demonstrate that the SF/HA composites exhibit better cell affinity than pure n-HA. However, SF content has no obvious effect on the cell affinity of n-SF/HA 20wt% SF/HA and 30wt% SF/HA show better os- teoblast proliferation.

关 键 词：羟基磷灰石 丝素蛋白含量 纳米晶 仿生矿化 细胞相容性 

分 类 号：TB321[一般工业技术—材料科学与工程]