玫瑰糠疹患者外周血单个核细胞中转录因子T-bet,GATA-3及Foxp3 mRNA的表达  

Expression of T-bet, GATA-3 and Foxp3 mRNA in Peripheral Blood Mononuclear Cells from Patients with Pityriasis Rosea

作　　者：张晓光[1] 李玉平[1] 王凌[1] 王爱学[1] 王大虎[1] 刘爽[1] 马喜兴[1] 马耀辉[1] 李艳玲[1] 

机构地区：[1]河北医科大学第二医院皮肤性病科,河北石家庄050000

出　　处：《中国皮肤性病学杂志》2012年第9期782-785,共4页The Chinese Journal of Dermatovenereology

基　　金：河北省卫生厅指导性课题(20100339)

摘　　要：目的探讨转录因子T-bet,GATA-3和Foxp3在玫瑰糠疹发病中的作用机制。方法采用SYBR GreenI实时荧光定量逆转录-多聚酶链反应技术检测20例玫瑰糠疹患者和22例正常人外周血单个核细胞中T-bet,GATA-3及Foxp3 mRNA的表达水平。结果玫瑰糠疹患者T-bet mRNA RQ值为(0.83±0.57),其表达水平高于正常对照组(0.52±0.36),两者比较差异有统计学意义(P<0.05)。玫瑰糠疹患者GATA-3及Foxp3 mRNA RQ值分别为(0.68±0.29),(0.92±0.61),其表达水平低于正常对照组的(1.22±1.02),(1.48±0.93),差异均有统计学意义(P均<0.05)。结论玫瑰糠疹患者存在T-bet/GATA-3升高,Foxp3降低,Th1细胞亚群优势表达可能性很大,细胞免疫异常可能在玫瑰糠疹发病机制中起着重要作用。Objective To explore the roles of transcription factors T-bet , GATA-3 and Foxp3 in pathogenesis of pityria- sis rosea （PR）. Methods SYBR Green 1 Real-time fluorescence quantitative RT-PCR was employed to de tect the expression of T-bet, GATA-3 and Foxp3 mRNA in peripheral Blood Mononuclear cells from 20 patients with PR and 22 healthy controls. Results The gene T-bet mRNA RQ values in PR group and the control group were （0.83±0.57） and （0.52 ±0.36）, and the expression＇level of T-bet mRNA in the PR group was higher than that in the control group （ P 〈 0.05 ）. The gene GATA-3 mRNA RQ values in PR group and the control group were （0.68 ± 0.29） and （ 1. 22 ±1.02）, and the expression level of GATA-3 mRNA in the PR group was lower than that in the control group（P 〈0.05）. The gene Foxp3 mRNA RQ val ues in PR group and the control group were （0.92 ± 0. 61 ） and （1.48 ±0. 93 ）, and the expression level of Foxp3 mRNA in the PR group was lower than that in the control group（P 〈 0.05）. Conclusion The ratio of T-bet/GATA-3 increases in patients with PR, while Foxp3 decreases. There is a high possibility that Thl cells subsets are predominant and the abnormality of cellular immunity may play an important role in the pathogenesis of PR.

关 键 词：玫瑰糠疹 T-BET GATA-3 FOXP3 

分 类 号：R758.64[医药卫生—皮肤病学与性病学]