威灵仙总皂苷体外抑制NB4细胞增殖及对CD11b、CD33表达的影响  

Effect of total saponin from clematis chinensis osbeck on proliferation and CD116/CD33 expression of NB4 cells in vitro

作　　者：黄莉[1] 李晓明[1] 黄纯兰[1] 

机构地区：[1]泸州医学院附属医院血液内科,四川泸州646000

出　　处：《泸州医学院学报》2012年第4期353-358,共6页Journal of Luzhou Medical College

基　　金：四川省卫生厅基金项目(070266);泸州市科技局基金项目(2007279)

摘　　要：目的:制备纯化的威灵仙总皂苷,体外作用于急性早幼粒细胞白血病NB4细胞,研究威灵仙总皂苷对NB4细胞分化的影响。方法:取对数生长期的NB4细胞,设置多个浓度梯度的威灵仙总皂苷药物组,观察用药后不同时间点NB4活细胞数,计算生长抑制率;Wright's-Giemsa染色计数法和NBT还原试验测定细胞分化率,透射电镜观察细胞形态学变化;流式细胞术检测CD11b/CD33表达情况。结果:与空白对照组比较,威灵仙总皂苷对NB4细胞有明显的生长抑制作用,且具有剂量-效应依赖关系;但具有统计学意义的诱导分化现象不明显。结论:威灵仙总皂苷可有效抑制NB4细胞增殖,但对NB4细胞无明显的诱导分化作用。Objective： To study the effect of total saponin from clematis chinensis osbeck on proliferation and differentiation of acute promyelocytic leukemia NB4 cell line in vitro.Methods：NB4 cells were treated with different concentration of total saponin for five days.The proliferation inhibition rates of NB4 cells induced by total saponin were calculated every day.Cell differentiation was demonstrated by Giemsa stain,transmission electron microscope（TEM） and NBT reduction assay at different time.The expressions of CD11b and CD33 induced by these drugs were measured by flow cytometry（FCM） at different time.Results： Compared with control group,clematis total saponin significantly inhibited the growth of NB4 cells dose dependently;but there was no statistical significance in cell differentiation.Conclusion： The total saponin can obviously inhibit the proliferation of NB4 cells,but has no significant role in cell differentiation.

关 键 词：威灵仙总皂苷 急性早幼粒细胞白血病 NB4细胞株 细胞分化 

分 类 号：R55[医药卫生—血液循环系统疾病]