阴道毛滴虫AP33基因的乳酸菌表达载体的构建  

Construction of the expression plasmid of T.vaginalis AP33 gene in lactic acid bacteria

作　　者：罗波[1] 段素群[2] 王光西[3] 陈文碧[3] 毛樱逾[3] 

机构地区：[1]泸州医学院生物化学教研室,四川泸州646000 [2]泸州医学院教务处,四川泸州646000 [3]泸州医学院病原生物学教研室,四川泸州646000

出　　处：《泸州医学院学报》2012年第4期379-381,共3页Journal of Luzhou Medical College

基　　金：四川省教育厅课题(11ZB227);泸州市科技局课题(泸市科[2007]16号)

摘　　要：目的:构建阴道毛滴虫AP33基因的乳酸菌表达载体。方法:提取阴道毛滴虫总RNA为模板,经RT-PCR得到AP33基因的PCR特异性产物,将其连接入乳酸菌分泌表达载体pVE5523质粒中,并转化入TOP10菌中保存。结果 :构建的AP33-pVE5523重组表达载体,经PCR鉴定正确;获得的目的基因片段经测序与GenBank公布的阴道毛滴虫AP33基因序列100%符合。结论:成功构建AP33-pVE5523表达载体,为进一步研究阴道毛滴虫AP33基因在乳酸菌中的表达打下基础。Objective： To construct the expression plasmid AP33-pVE5523.Methods： The total RNA extracted from the trophozoites of T.vaginalis was used as template for the amplification of AP33 gene by using RT-PCR.The amplified products were ligated with expression plasmid pVE5523 of lactic acid bacteria（LAB）.Then the recombination product AP33-pVE5523 was preserved in TOP10 bacteria.Results：The recombination product AP33-pVE5523 checked by PCR was correct.The DNA sequence analysis revealed that the homology with the gene of T.vaginalis adhesion protein AP33-1 was 100%.Conclusion： The expression plasmid AP33-pVE5523 was constructed successfully,which provides basis for the reaserch of AP33 expression in LAB.

关 键 词：阴道毛滴虫 黏附蛋白AP33 乳酸菌表达载体 构建 

分 类 号：R392.7[医药卫生—免疫学]