糖基化终产物对人牙龈成纤维细胞2种基质金属蛋白酶表达的影响  

Effects of advanced glycation end products on the expression of matrix matelloproteinases in human gingival fibroblasts

作　　者：邓雨泉[1] 付云[1] 李华菁[1] 

机构地区：[1]中山大学光华口腔医学院.附属口腔医院牙周病科,广东广州510055

出　　处：《广东牙病防治》2012年第8期404-408,共5页Journal of Dental Prevention and Treatment

基　　金：广东省医学科学技术研究基金(B2010119)

摘　　要：目的研究糖基化终产物(advanced glycation end products,AGEs)对人牙龈成纤维细胞(human gingivalfibroblasts,HGF)表达基质金属蛋白酶-1(matrix matelloproteinase-1,MMP-1)和基质金属蛋白酶-8(matrix matallo-proteinase-8,MMP-8)的影响,探讨糖尿病加速牙周炎发展的可能机制。方法将培养的HGF随机分成6组:空白对照组只加入培养液;阴性对照组仅加入含50μg/mL人血清白蛋白(human serum albumin,HSA)的培养液;4个含AGE-HSA的实验组分别加入含0.5、5、50、100μg/mL AGE-HSA的培养液。培养24、48、72 h后,分别应用酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA)和实时定量聚合酶链反应(real-time quantitativepolymerase chain reaction,QPCR)检测细胞MMP-1、MMP-8的蛋白和mRNA表达。结果 100μg/mL AGE-HSA组培养24、48和72 h后,MMP-1水平明显高于阴性对照组、空白对照组及0.5μg/mL AGE-HSA组,差异具有统计学意义(P<0.05)。各浓度AGE-HSA组MMP-1 mRNA水平均显著高于阴性对照组,差异有统计学意义(P<0.05)。各浓度AGE-HSA组MMP-8水平与阴性对照组相比差异均无统计学意义(P>0.05),MMP-8 mRNA表达均为阴性。结论 AGEs可能通过促进HGF合成MMP-1,介导胶原降解,从而加重糖尿病患者的牙周组织破坏,尚不能说AGEs影响MMP-8的表达。Objective To explore the role of （advanced glyeation end products（AGEs）in modulating the expression of matrix matilloproteinase-1（MMP-1）and matrix matelloproteinase-8（MMP-8）in human gingival fibroblasts （HGF）.Methods The cultured HGF cells were randomly divided into six groups：blank control group.HSA50 μg/mL stimulation group（negative control group）and AGE-modified human serum albumin（AGE-HSA）0.5,5.50and 100μg/mL stimulation groups.After 24,48and 72 hours of culturing,MMP-1and MMP-8 protein and mRNA expression were detected by enzyme-lindked immunosorbent assay （ELISA）and real-time quantitative polymerase chain reaction（QPCR）method respectively.Results At 24,48and 72 hours,the level of MMP-1 in 100μg/mL AGE-HSA group was significantly gradually higher than those in HSA group,blank control group and 0.5μg/mL AGE-HSA group（P〈0.05）.The level of MMP-1 mRNA in each concentration AGE-HSA group was significantly higher than that in HSA group（P〈0.05）.There was no statistically significant difference between the level of MMP-1 in each concentration AGE-HSA group and that in HSA group（P〈0.05）.MMP-8 mRNA expression in each concentration AGE-HSA group was negative.Conclusion SGEs may promote synthesis of MMP-1.SGEs may play an important role in periodontal tissue destruction by promoting extracellular collagen degradation.AGEs had no significant effeet on the expression of MMP-8 in eultured HGF according to our results.

关 键 词：晚期糖基化终末产物 人牙龈成纤维细胞 基质金属蛋白酶 

分 类 号：R349.51[医药卫生—基础医学]