类模板分子印迹整体柱识别人血清中甲氨蝶呤的研究  

Study on recognition of methotrexate in human serum by molecularly imprinted monolithic column with an analogue template employed

作　　者：张红武[1] 史美丽[2] 李雪美[1] 陈星宇[1] 卢绮雯[1] 蔡冰[1] 

机构地区：[1]广东药学院药科学院,广东广州510006 [2]广东药学院附属门诊部,广东广州510224

出　　处：《广东药学院学报》2012年第3期268-272,共5页Academic Journal of Guangdong College of Pharmacy

基　　金：中国科学院活体分析化学重点实验室开放基金资助项目(ACL201202)

摘　　要：目的制备分子印迹整体柱,实现对人血清中甲氨蝶呤(MTX)的选择性富集,为其HPLC法分析提供便利。方法以三聚氰胺为类模板,甲基丙烯酸为功能单体,二甲基丙烯酸乙二醇酯为交联剂,聚乙二醇和甲醇为致孔剂,采用原位聚合法在不锈钢空色谱柱管内合成了分子印迹(MIP)整体柱。以该MIP柱为色谱柱,通过HPLC法分析考察其选择性识别MTX的效果。结果在依次经过甲醇-水(体积比1∶9)和乙腈-水-乙酸(体积比96∶3.5∶0.5)2种流动相的洗脱后,MTX依然能在MIP柱上保留而不出峰,人血清样中的基质干扰成分则能被完全清除,最后吸附在MIP柱上的MTX可用甲醇洗脱出峰。结论在选定的最佳色谱流动相条件下,制备的MIP整体柱对MTX显示良好的选择性识别作用,同时可将血清中的基质干扰组分除去。该MIP柱有望用于在线或离线选择性富集人血样中的MTX,为RP-HPLC法提供了便利。Objective To prepare a molecularly imprinted monolithic column for selectively enriching methotrexate（MTX） in human serum in order to facilitate the RP-HPLC analysis.Methods Melamine was employed as an analogue template to prepare the molecularly imprinted monolithic column by in-situ polymerization.Methacrylic acid was used as a function monomer and ethylene glycol dimethacrylate as a cross linker during the polymerization process.The recognition ability of the imprinted monolithic column towards MTX was evaluated by HPLC analysis.Results The matrix impurities in human serum could be eliminated and MTX was retained on MIP column during consecutive elution with methanol-water（1∶9） and acetonitrile-water-acetic acid（96∶3.5：0.5）.Finally,MTX was obtained through the elution process of methanol for HPLC analysis.Conclusion The prepared MIP monolithic column showed good selective recognition capacity towards MTX under the optimal conditions of mobile phase,which may be applied in online or off-line manner to selectively enrich MTX in human serum,and clear the matrix impurities to provide good MTX fraction for RP-HPLC analysis.

关 键 词：分子印迹聚合物 整体柱 类模板 选择性富集 高效液相色谱法 甲氨蝶呤 人血清 

分 类 号：R96[医药卫生—药理学]