BMP-4对人毛囊外根鞘细胞增殖和迁移的影响  

The effect of BMP-4 on the proliferation and migration of human hair follicle outer root sheath cell

作　　者：顾华[1] 李坤杰[2] 凃颖[1] 刘玲[1] 何黎[1] 

机构地区：[1]昆明医科大学第一附属医院皮肤科,云南省皮肤性病研究所,昆明650032 [2]福建医科大学第二附属医院皮肤科,泉州362000

出　　处：《皮肤病与性病》2012年第4期187-189,共3页Dermatology and Venereology

基　　金：云南省社会发展科技计划项目;编号:2007CA012

摘　　要：目的观察BMP-4对体外培养的人毛囊外根鞘细胞增殖和迁移的影响。方法用含不同浓度BMP-4的MSCM培养基培养毛囊外根鞘细胞48小时后行MTT检测毛乳头细胞的增殖情况;将铺满90%培养皿底的毛囊外根鞘细胞划痕后以含不同浓度BMP-4的MSCM培养基培养,分别于0h,8h,24h和48h测量毛囊外根鞘细胞爬行的距离。结果与其他浓度相比,10μg/mlBMP-4明显促进毛囊外根鞘细胞的增殖(P<0.05),不同浓度BMP-4均促进毛囊外根鞘细胞的迁移,48小时10μg/ml浓度BMP-4的创面愈合指数达到100%(P<0.05)。结论 BMP-4对于毛囊形成及发育具有重要意义。Objective To investigate the effect of BMP-4 on the proliferation and migration of human hair follicle outer root sheath cell. Methods The human hair follicle outer root sheath ceils were cultured and treated with BMP-4 of different concentration, and MTT method was used to evaluate the proliferation ability of ceils. The hair follicle outer root sheath cells were cultured in 12-well plates and treated with mitomycin C, then a single scratch wound was made in each well; and the cells were treated with BMP-4 of different concen- tration. The distance of cell migration was measured in different time point. Results compared with control group, BMP-4 at the concen- tration of 10ug/ml promoted the proliferation and migration of human hair follicle outer root sheath cells. Conclusion BMP-4 play an important role in hair follicle development and growth.

关 键 词：毛囊外根鞘细胞 BMP-4 增殖 迁移 

分 类 号：R334.5[医药卫生—人体生理学]