3,4-二羟基苯乙酸甲酯对肠道病毒71型在人横纹肌肉瘤细胞中复制的抑制  

Methyl 3,4-dihydroxyphenylacetate prevents enterovirus 71 proliferation in rhabdomyosarcoma cells

作　　者：张迎秋[1] 赵春晖[1] 杨倬[2,3] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116029 [2]中国科学院微生物研究所,病原微生物与免疫学重点实验室,北京100101 [3]清华大学生命科学学院,北京100084

出　　处：《药学学报》2012年第9期1257-1260,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(21102067);辽宁省教育厅资助项目(2009A413)

摘　　要：肠道病毒71型（enterovirus71，EV71）属于小RNA病毒科（Picrornaviridae）肠道病毒属（Enterovirus，EV），是手足口病的主要病原体。手足口病至今己在世界各地爆发流行，在亚洲国家流行更为严重。儿童感染EV71可引起发热、口腔黏膜溃疡性疱疹和四肢末端水疱样皮疹，This article presented the inhibitory activity of methyl 3, 4-dihydroxyphenylacetate on the enterovirus 71 （EV71） infection. The EV71 VP1 capsid protein expression levels were analyzed with Western blotting. Results revealed that the compound is able to inhibit EV71 replication in rhabdomyosarcoma （RD） cells. After being incubated with the compound at a concentration of 0.01 μg μL-1 for 48 h, the level of EV71 vpl mRNA in RD cells decreased by （76.83±2.47）%. The cytotoxic activity of the compound was evaluated against RD cells by a MTT assay. The results showed that the compound had low toxicity with a CC50 of 0.072 6μg·μL-1. These findings suggest that methyl 3, 4-dihydroxyphenylacetate is a novel compound for antiviral therapies against EV71, which merited further investigation.

关 键 词：肠道病毒71型 3 4-二羟基苯乙酸甲酯 抗病毒作用 

分 类 号：R963[医药卫生—微生物与生化药学]