Comparison of intestinal bacterial communities in grass carp,Ctenopharyngodon idellus,from two different habitats  

作　　者：倪加加 余育和 张堂林 高雷 

机构地区：[1]Key Laboratory of Biodiversity and Conservation of Aquatic Organisms,Institute of Hydrobiology,Chinese Academy of Sciences [2]Graduate University of Chinese Academy of Sciences [3]State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences

出　　处：《Chinese Journal of Oceanology and Limnology》2012年第5期757-765,共9页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Basic Research Program of China(973 Program)(No.2009CB118705);the National Natural Science Foundation of China(No.30970358)

摘　　要：The intestinal bacteria of vertebrates form a close relationship with their host.External and internal conditions of the host,including its habitat,affect the intestinal bacterial community.Similarly,the intestinal bacterial community can,in turn,influence the host,particularly with respect to disease resistance.We compared the intestinal bacterial communities of grass carp that were collected from farm-ponds or a lake.We conducted denaturing gradient gel electrophoresis of amplified 16S rRNA genes,from which 66 different operational taxonomic units were identified.Using both the unweighted pair-group method with arithmetic means clustering and principal component analysis ordination,we found that the intestinal bacterial communities from the two groups of pond fish were clustered together and inset into the clusters of wild fish,except for DF-7,and there was no significant correlation between genetic diversity of grass carp and their intestinal bacterial communities(Mantel one-tailed test,R=0.157,P=0.175).Cetobacterium appeared more frequently in the intestine of grass carp collected from pond.A more thorough understanding of the role played by intestinal microbiota on fish health would be of considerable benefit to the aquaculture industry.The intestinal bacteria of vertebrates form a close relationship with their host. External and internal conditions of the host, including its habitat, affect the intestinal bacterial community. Similarly, the intestinal bacterial community can, in turn, influence the host, particularly with respect to disease resistance. We compared the intestinal bacterial communities of grass carp that were collected from farm-ponds or a lake. We conducted denaturing gradient gel electrophoresis of amplified 16S rRNA genes, from which 66 different operational taxonomic units were identified. Using both the unweighted pair-group method with arithmetic means clustering and principal component analysis ordination, we found that the intestinal bacterial communities from the two groups of pond fish were clustered together and inset into the clusters of wild fish, except for DF-7, and there was no significant correlation between genetic diversity of grass carp and their intestinal bacterial communities （Mantel one-tailed test, R=0.157, P=0.175）. Cetobacterium appeared more frequently in the intestine of grass carp collected from pond. A more thorough understanding of the role played by intestinal microbiota on fish health would be of considerable benefit to the aquaculture industry.

关 键 词：denaturing gradient gel electrophoresis grass carp HABITAT intestinal bacterial community 

分 类 号：S917.4[农业科学—水产科学]