炙山茱萸高效液相色谱指纹图谱研究  

HPLC fingerprint of Corni Fructus Praeparata Cum Melle

作　　者：高新彪[1,2] 孙磊[2] 乔善义[2] 王金辉[1] 高松 车彦忠 

机构地区：[1]沈阳药科大学中药学院基于靶点的药物设计与研究教育部重点实验室,沈阳110016 [2]军事医学科学院毒物药物研究所植物化学室,北京100850 [3]河南宛西制药有限公司,南阳473000

出　　处：《国际药学研究杂志》2012年第4期330-336,共7页Journal of International Pharmaceutical Research

基　　金：国家"重大新药创制"科技重大专项课题(2011ZX09201-201-13;2009ZX09301-002);国家科技支撑计划课题(2006BAI08B03-08)

摘　　要：目的建立炙山茱萸高效液相色谱(HPLC)指纹图谱。方法色谱柱为Dikma Diamonsil C18色谱柱(250 mm×4.6 mm,5μm);以乙腈-水(含0.08%磷酸)为流动相梯度洗脱;柱温:40℃;流速:0.8 ml/min(0～50 min),0.8～1.0 ml/min(50～60 min);检测波长:276 nm(0～15 min),236 nm(15～46 min),276 nm(46～60 min);进样量:20μl。采用Q-TOF-MS-IDA-MS/MS方法对22个共有峰中的16个色谱峰进行了定性鉴别。采用该方法测定了10批不同来源的炙山茱萸。结果该方法精密度、稳定性和重复性良好。结论该研究为炙山茱萸的全面质量评价奠定了基础。Objective To establish the fingerprint of Corni Fructus Praeparata Cum Melle by HPLC.Methods The chromatographic fingerprint was obtained with Dikma Diamonsil C18 column（250 mm × 4.6 mm,5 μm） and a gradient elution with the mobile phase consisted of acetonitrile and 0.08% phosphoric water.The column temperature was maintained at 40 ℃,and the flow rate was 0.8 ml/min（0-50 min）,0.8-1.0 ml/min（50-60min）.The detection wavelength was 276 nm（0-15 min）,236 nm（15-46 min） and 276 nm（46-60 min）.The injection volume was 20 μl.Sixteen chromatographic peaks were identified by Q-TOF-MS-IDA-MS/MS method.Ten batches of Corni Fructus Praeparata Cum Melle were determined.Results Good precision,stability and repeatability were obtained.Conclusion The studies lay a foundation for a comprehensive quality evaluation of Corni Fructus Praeparata Cum Melle.

关 键 词：炙山茱萸 高效液相色谱 指纹图谱 Q-TOF-MS-IDA-MS MS 质量评价 

分 类 号：R284[医药卫生—中药学]