解毒通络方对兔心肌缺血再灌注损伤心肌酶的影响  

Effect of dispelling toxins and dredging collaterals meridians and collaterals on myocardial enzyme in rabbit with myocardial ischemia/reperfusion injury

作　　者：杨健 

机构地区：[1]河北省唐山市中医医院针灸科,河北唐山063000

出　　处：《河北中医》2012年第7期1066-1067,1074,共3页Hebei Journal of Traditional Chinese Medicine

基　　金：河北省中医药管理局2008年度中医药;中西医结合科研计划课题(编号:2008043)

摘　　要：目的研究解毒通络方对实验性兔心肌缺血再灌注损伤(MIRI)血清心肌酶的影响。方法将32只日本大耳白兔随机分为4组,即空白对照组、模型组、解毒通络方低剂量组及解毒通络方高剂量组,每组各8只。解毒通络方低、高剂量组分别予3.16、6.32 g/(kg.d)生药,溶液量15 mL灌胃给药;空白对照组和模型组分别予等容积0.9%氯化钠注射液灌胃给药。均连续5 d。然后模型组、解毒通络方低剂量组及解毒通络方高剂量组行左冠状动脉前降支穿线,结扎,30 min后打开结扎实现再灌注,空白对照组只穿线,不结扎。测定各组天冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、肌酸激酶(CK)及肌酸激酶同工酶(CK-MB)水平。结果模型组、解毒通络方低剂量组及解毒通络方高剂量组AST、LDH、CK及CK-MB与空白对照组比较均明显升高(P<0.05)。解毒通络方低剂量组及解毒通络方高剂量组AST、LDH、CK及CK-MB与模型组比较均明显降低(P<0.05,P<0.01)。解毒通络方高剂量组AST、LDH、CK及CK-MB与解毒通络方低剂量组比较略低,但无统计学意义(P>0.05)。结论解毒通络方可降低兔MIRI心肌酶,对心肌具有保护作用。Objective To investigate the effects of dispelling toxins and dredging collaterals meridians and collaterals on myocardial enzyme in rabbit with myocardial ischemia/reperfusion injury. Methods 32 Japanese white rabbits were randomly divided into four groups, sham operation group, myocardial ischemia group, dispelling tox- ins and dredging collaterals therapy low dosage group （ group C）, dispelling toxins and dredging collaterals therapy high dosage group （ group D）. There were 8 rabbits in each group. Low dose group and the high dose group were 3. 16,6.32 g/（ kg · d） of crude drug. Liquid volume of 15 mL was administered orally. The equal volume of 0.9% so- dium chloride injection was administered orally in the blank control group and model group for 5 days of continuous administration. The threading,ligation of left anterior descending branch was carried out in dredging collaterals group B, C and D. Open ligation achieved reperfusion after 30 min, the blank control group only wear line was not ligated. The levels of AST,LDH,CK and CK -MB were measured in each group. Results The levels of AST,LDH,CK,CK -MB in group B, C and D were significantly increased in comparison with those in group A （P 〈 O. 05）. The levels of AST,LDH,CK,CK -MB in group C and D were significantly decreased in comparison with those in group B （P 〈 0.05 ,P 〈0.01 ）. The levels of AST,LDH,CK,CK - MB in group D were lower than those in group C,but there was no significance （P 〉 0.05 ）. Conclusion Dispelling toxins and dredging collaterals therapy may reduce injury in myocardial cell,it has protective effect on myocardial ischemiaYreperfusion.

关 键 词：心肌缺血 心肌再灌注损伤 解毒剂(中药) 通络 模型 动物 兔 L-乳酸脱氢酶 

分 类 号：R542.205.31[医药卫生—心血管疾病] R285.5[医药卫生—内科学]