衣藻属的系统发育分析——基于形态形状和nrDNA ITS序列  

PHYLOGENETIC ANALYSIS ON GENUS CHLAMYDOMONAS BASED ON MORPHOLOGICAL CHARACTERS AND nrDNA ITS SEQUENCE

作　　者：张弛[1] 胡鸿钧 李中奎 李夜光 钟扬[1] 

机构地区：[1]中国科学院武汉植物研究所经济微藻研究室,武汉430074

出　　处：《武汉植物学研究》2000年第3期189-194,共6页Journal of Wuhan Botanical Research

基　　金：国家自然科学基金资助项目!(No.3 9870 0 63 )

摘　　要：通过实验分析莱茵衣藻 ( Chlamydomonas reinhardtii) 1个种和互连网获得衣藻属 1 5个种及丝藻属 1个种 ( Ulothrix zonata) ,共 1 7个种的 nr DNA ITS序列 ,并以 U.zonata为外类群 ,采用计算机分析软件包对其进行分析及构建分子系统发育树图。同时以 1 2个传统分类性状 ,对此 1 6种衣藻构建数据矩阵 ;以 U.zonata动孢子的相应性状为外类群原始性状 ,用Wagner法在计算机上对其进行分枝分析 ;然后比较并分析分子系统树和表征性状分支分析树的异同。初步尝试以 ITS分子序列系统发育分析作为传统性状分析的补充来研究衣藻种间的亲缘关系。The sequences of ITS regions and 5.8S coding region of nuclear ribosomal DNA from 16 species of the genus Chlamydomonas were determined and analysed.Based on the ITS sequences,the phylogentic tree of these species was constructed using the nrDNA ITS sequence of Ulothrix zonata as the outgroup.And on the basis of 12 morphological characters for study in the 16 species,phylogenetic analysis was performed and then Wagner method was used to construct the phylogenetic tree.Finally the two phylogenetic trees were compared and the phylogenetic affinity between the 16 species of the genus Chlamydomonas was analysed.The close affinity between tree pairs of species:C.incerta with C.reinhardtii;C.geitleri with C.noctigarna;C.asymmetrica with C.mexicana is supported by both nrDNA ITS analysis and the morphological characters analysis.However,the result shows that there is significant difference between the phylogenetic tree from the nrDNA ITS sequences analysis and the phylogenetic tree from morphological characters analysis.The two phylogenetic trees don't agree with each other as a whole,and the possible reason for the difference is discussed.

关 键 词：衣藻属 系统发育 nrDNAITS 分支分析 表征性状 

分 类 号：Q949.212[生物学—植物学]