抗癌方对裸鼠移植性人肝癌细胞形态学及P21^(Waf1/Cip1)蛋白表达的影响  

The Evaluation of TCM Anticancer Prescription to the Morphology and Expression of P21^(Waf1/Cip1) Protein of the Restraining Tumor from Growing in Tumor Bearing Nude Mice

作　　者：郭振球[1] 朱梅菊[1] 

机构地区：[1]湖南中医学院中医诊断学研究所,中国湖南长沙410007

出　　处：《生命科学研究》2000年第2期161-166,共6页Life Science Research

基　　金：国家自然科学基金资助项目!( 3 9770 91 7)

摘　　要：为了探索中药抗癌方的疗效机理 ,采用裸鼠移植性人肝癌细胞 ,分中药组 ( TCM)、环磷酰胺 ( CTX)组和空模组对照治疗试验 .结果发现 :中药组中癌细胞的形态改变以凋亡为主 ,而 CTX组中癌细胞的形态改变主要为坏死 .在可疑凋亡细胞处 ,光镜常见 P2 1 Waf1/ Cip1表达 .其疗效机理与诱导 Hep G2 凋亡 ,提高 P2 1 Waf1/ Cip1蛋白表达有关 .In order to search the therapeutic effectiveness mechanism of TCM anticancer presription, we used the restraining tumor from growing in tumor bearing nude mice which were divided into 3 groups (TCM group, CTX group and control group ) before therapeutic experiments. The results are below: The morphological changes of the tumor cells in the TCM group are mainly apoptosis but that in the CTX group are necrosis. The expression of P21 Waf1/Cip1 in the apoptosis cells is commonly seen through optical microscope. Maybe the therapeutic effectiveness mechanism is related to inducing HepG 2 apoptosis and improving the expression of P21 Waf1/Cip1 protein.

关 键 词：中药抗癌方 细胞凋亡 疗效机理 P21蛋白表达 

分 类 号：R286.91[医药卫生—中药学] R289.5[医药卫生—中医学]