复方甘草酸苷对大鼠急性肝衰竭保护机制的研究  

Efficacy of protective mechanism of compound glycyrrhizin on acute hepatic failurein rats

作　　者：裴旭东[1] 翟玉峰[1] 张怀宏[1] 

机构地区：[1]河南省南阳市中心医院,河南南阳473009

出　　处：《实用药物与临床》2012年第8期464-466,共3页Practical Pharmacy and Clinical Remedies

摘　　要：目的探讨甘草酸苷对大鼠急性肝衰竭(AHF)的保护机制。方法将90只Wistar大鼠随机分为3组:正常对照组(对照组)、模型组(AHF组)、治疗组(甘草酸苷组),静脉注射硫代乙酰胺(TAA)600 mg/kg,2次,每次间隔24 h,复制AHF动物模型。治疗组动物除予TAA外,于实验前3 d至实验结束分别静滴甘草酸苷注射液2 mL/100 g,对照组和AHF组同时皮下注射0.9%氯化钠液1 mL/100 g。AHF组、治疗组于第2次注射TAA后24 h,随机各取8只,治疗中分时段取大鼠腹主动脉血检测肝功能、PTA、血清内毒素及多种炎性因子水平。2周后,取肝组织,用10%甲醛液固定,检测肝细胞增殖细胞核抗原阳性率(PCNA)。结果与AHF组、对照组相比,治疗中,治疗组血清ALT水平降低(P<0.01);治疗2周后,治疗组血清总胆红素水平(同比例)降低,而血浆凝血酶原活动度(PTA)均值较高(P<0.01);治疗后各时相点,治疗组TNF-α水平较低(P<0.01);治疗2周后,治疗组患者血浆内毒素及IL-6水平显著降低(P<0.01)。治疗2周后,治疗组PCNA阳性细胞表达显著高于对照组(P<0.01)。结论甘草酸苷对急性肝衰竭大鼠模型具有保护作用,其机制可能与降低血浆内毒素及细胞因子水平、促进肝组织PCNA表达有关。Objective To study the protective mechanism of compound glycyrrhizin（CG） on acute hepatic failure（AHF） in rats.Methods 90 rats were randomly divided into normal group,model group and treatment group（compound glycyrrhizin）.Thioacetamide（TAA） 600 mg/kg was given（iv.） once daily for 2 times,and model of AHF was made.Treatment group was given CG 2 mL/100 g（iv.） at 3 d before and after treatment besides TAA,while control group and AHF group were given NS 1 mL/100 g（ih.）.The ALT,TBIL,PTA,endotoxin and many kinds of inflammatory factor level were measured.The hepatic tissue was taken and fixed into 10% formalin after 2 weeks,and the masccline rate of proliferation cell nuclear antigen was detected.Results Compared with AHF group and control group,serum ALT levels in treatment group were significantly lower（P0.01）;The average level of serum total bilirubin（TBIL） in treatment group was lower,and the average level of serum plasma prothrombin activity（PTA） was higher after 2 weeks（P0.01）;The level of TNF-a in treatment group was lower during treatment（P0.01）.The levels of plasma endotoxin and interleukin-6 in treatment group were significantly lower after 2 weeks（P0.01）.The masccline rate of proliferation cell nuclear antigen in treatment group was higher than those of control group after 2 weeks（P0.01）.Conclusion CG can protect AHL of rat model,and the mechanism could be concerned with decreasing levels of endotoxin and cytokine,promoting express of proliferation cell nuclear antigen.

关 键 词：急性肝衰竭 内毒素 细胞因子 增殖细胞核抗原 甘草酸苷 

分 类 号：R285.5[医药卫生—中药学]