用AFLP技术和16SrDNA PCR-RFLP分析毛苜蓿根瘤菌的遗传多样性  

GENETIC DIVERSITY OF RHIZOBIA OF MEDICAGO EDGEWORTHII BY AFLP AND RFLP ANALYSIS

作　　者：冯瑞华[1] 

机构地区：[1]中国农业科学院土壤肥料研究所,北京100081

出　　处：《微生物学报》2000年第4期339-345,共7页Acta Microbiologica Sinica

基　　金：联合国教科文组织的短期进修基金

摘　　要：采用选择性扩增片断长度多态性 (简称AFLP)DNA指纹技术对采自我国云南省与西藏交界的高山地区的野生型豆科植物毛苜蓿根际土样分离的 2 91株毛苜蓿 (Medicagoedge worthii)根瘤菌进行遗传多样性的研究。从AFLP图谱中 ,揭示出毛苜蓿根瘤菌有较显著的遗传多样性 ,从 2 91株中选择出 90个代表株用计算机进行树状图的分析。结果表明 ,所分析的菌株在 79%的相似性水平上聚类成 3个群。对这 90个代表株进行多聚酶链反应 (PCR)扩增的 1 6SrDNA的 4种限制性内切酶长度多态 (简称 1 6SrDNAPCR RFLP)分析 ,得出 2个不同的 1 6SrDNAPCR RFLP类型的菌株。分别选出这 2个类型的代表菌株与各种根瘤菌的参比菌株进行 1 6SrDNAPCR RFLP分析 ,再进行树状图的分析 ,初步得出了它们在根瘤菌系统分类中的地位。分析结果表明 :毛苜蓿根瘤菌与根瘤菌属中的Rhizobiummongolense的相似性很高。Two hundred and ninety one isolates of rhizobia sampled from root nodules of \%Medicago edgeworthii\% which came from Yun Nan Province in china were analyzed by amplified restriction fragment polymorphism(AFLP) technique. According to the AFLP banding patterns, the results showed most of these isolates were genetically diversity. Ninety isolates were selected as representing the diversity among the 291 isolates. The 90 isolates were clustered into three groups at the level of similarity of 79% by computer analysis of the data. Additional representative isolates and reference strains were identified by restriction fragment length polymorphism (RFLP) analysis of PCR\|Amplified 16S rDNA. Two different 16S rDNA PCR\|RFLP types were found, which indicated that the isoltes were phylogentically closely related to \%Rhizobium mongolense.\%

关 键 词：AFLP 16SrDNA 毛苜蓿根瘤菌 遗传多样性 牧草 

分 类 号：Q933[生物学—微生物学]