禾谷多粘菌(Polymyxa graminis)侵染及传毒体系的研究  

DEVELOPMENT OF A SYSTEM FOR POLYMYXA GRAMINIS INFECTION AND TRANSMISSION OF WHEAT YELLOW MOSAIC VIRUS\+*

作　　者：田兆丰 陈笑瑜 朱坤 于嘉林[1] 刘仪[1] 

机构地区：[1]中国农业大学农业生物技术国家实验室,北京100094

出　　处：《微生物学报》2000年第4期352-358,共7页Acta Microbiologica Sinica

基　　金："8 63"计划资助项目! ( 863 1 0 1 0 4 0 1 0 5)

摘　　要：在人工气候箱内 ,以小麦为寄主建立了专性寄生禾谷多粘菌 (Polymyxagraminis)的侵染体系 ,使P .graminis能够快速大量繁殖 ,生活史缩短为 1 3～ 1 5d。简化了单孢子堆分离以及病根表面消毒等分离纯化方法 ,对接种菌源材料、寄主苗龄、温度、pH值及营养液成分等影响因素进行了测定 ,优化完善了P .graminis的砂培条件。建立了针对小麦黄花叶病毒(wheatyellowmosaicvirus,WYMV)的稳定高效室内传毒体系。在此体系上 ,真菌传带病毒的效率可达 70 % ,机械接种病毒后的小麦显症时间可缩短至 30d左右。In growth chambers, a system was developed for infection of \%Polymyxa graminis\% to wheat(\%Triticum aestivum\% L.), which is an obligate parasite. By the system, the fungus could be propagated in large quantity rapidly and the life cycle of P.graminis in wheat was observed in a period of 13 to 15 days. The methods for separating P.graminis as single cystosori from cultured wheat roots or dried roots were simplified. By tests for the factors affecting on P.graminis infection, including inocula types, seedling age of host plants, temperatures, pH value and nutrient contents etc., the system was modified to more perfection. With an integration of optimal temperature and other elements in the system, which affect on infection and development of P.graminis , wheat yellow mosaic virus(WYMV) was transmitted constantly and efficiently by the fungal vector. In average, 70% of wheat plants inoculated by the viruliferous fungi could infected by WYMV and the typical symptom of wheat mosaic disease would appear at 30 days post\|inoculation mechanically.

关 键 词：禾谷多粘菌 侵染 真菌传毒 植物病害 

分 类 号：S432.42[农业科学—植物病理学]