大线性质粒pHZ1000,pHZ1001在链霉菌种间的接合转移  

TRANSFER OF TWO LARGE LINEAR PLASMIDS pHZ1000 AND pHZ1001 BY CONJUGATION FROM STREPTOMYCES T8-4 TO S.LIVIDANS ZX1

作　　者：赵书春[1] 周秀芬[2] 邓子新[2] 

机构地区：[1]中国科学院微生物研究所,北京100080 [2]华中农业大学生命科技学院,武汉430070

出　　处：《微生物学报》2000年第4期435-439,共5页Acta Microbiologica Sinica

基　　金：国家自然科学基金!资助项目 ( 3 9770 3 96和 3 983 0 2 1 0 )

摘　　要：Using pulsed\|field gel electrophoresis (PFGE),two indigenous large plasmids were isolated from Streptomyces T8 4.Two dimentional PFGE revealed that both plasmids were linear molecules.By parallel electrophoresis with linear plasmids of known sizes,the two linear plasmids were estimated to be approximately 230kb and 90kb,which were designated as pHZ1000 and pHZ1001,respectively.Both plasmids could be transferred into S.lividans ZX1 by conjugation,which is detectable by “pock”formation.Five S.lividans ZX1 derivatives which carry one or two plasmids were isolated and characterized by Southern hybridization and PFGE.Using pulsed\|field gel electrophoresis (PFGE),two indigenous large plasmids were isolated from Streptomyces T8 4.Two dimentional PFGE revealed that both plasmids were linear molecules.By parallel electrophoresis with linear plasmids of known sizes,the two linear plasmids were estimated to be approximately 230kb and 90kb,which were designated as pHZ1000 and pHZ1001,respectively.Both plasmids could be transferred into S.lividans ZX1 by conjugation,which is detectable by “pock”formation.Five S.lividans ZX1 derivatives which carry one or two plasmids were isolated and characterized by Southern hybridization and PFGE.

关 键 词：大线性质粒 接合转移 链霉菌 基因工程载体 

分 类 号：Q933[生物学—微生物学]