抗稻瘟病水稻资源抗性基因Pita、Pib、Pi9以及Pikm的分布研究  

Distribution Research of Blast Resistance Genes Pita,Pib,Pi9 and Pikm in Blast-Resistant Rice Resources

作　　者：戴小军[1] 杨远柱 周亮[1] 梁满中[1] 胡小淳 陈良碧[1] 

机构地区：[1]湖南师范大学生命科学学院,中国湖南长沙410081 [2]隆平高科亚华种业科学院,中国湖南长沙410119

出　　处：《生命科学研究》2012年第4期340-344,356,共6页Life Science Research

基　　金：湖南省自然科学基金资助项目(10JJ3092);教育部博士点教师基金资助项目(20094306120005);国家863子课题(2010AA101305);湖南省重大攻关科技计划专项(2009FJ1004-1)

摘　　要：利用抗稻瘟病水稻资源品种杂交,聚合多个抗性基因是培育持久抗稻瘟病水稻新品种的主要育种途径.利用分子标记技术对水稻抗性资源进行基因型鉴定是分子辅助聚合育种的基础.通过以亚华种业科学院稻瘟病病圃抗病水稻资源为材料,利用特异性分子标记对Pi9、Pita、Pib以及Pikm基因在水稻抗稻瘟病资源的分布进行了鉴定,初步建立了抗性基因数据库.同时对抗性基因及与抗性反应的相关性进行了探讨,结果表明以Pi9为主效基因,同时聚合Pita和Pib抗性基因能提高持久抗稻瘟病能力.Gene pyramiding is an effective approach for the improvement of rice varieties with durable resis-tance to blast by crossing among some blast-resistant rice varieties.To blast-resistant rice resource,the foun-dation of marker-assisted selection（MAS） is resistance genotype identification with molecular markers.Some rice cultivars resistant to rice blast were selected by certifying with rice blast plot.Meanwhile,specific molecular markers were used to test the blast resistance genes Pi9,Pita,Pib and Pikm in the cultivars and the database contained resistant gene were established initially.Furtherly,the correlation between resistant gene and the disease reactions was discussed,and the result indicated that the durable blast-resistant ability would be improved when Pi9 was a major gene polymerized with Pita,Pib.

关 键 词：稻瘟病 抗性基因 分子标记 基因聚合 

分 类 号：S332.2[农业科学—作物遗传育种]