松针褐斑病菌毒素处理后湿地松组培苗PAL、PPO、SOD活性变化研究  

Study on PAL,PPO,SOD Activities of Tissue Cultured Plantlets of Slash Pine Treated with Lecanosticta acicola

作　　者：程方[1] 叶建仁[1] 刘戈[1] 安会翠[1] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《林业科学研究》2012年第4期521-525,共5页Forest Research

基　　金：国家林业局"948"项目

摘　　要：为研究抗松针褐斑病菌在湿地松子代组培苗体内的苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、超氧化物歧化酶(SOD)的活性变化与其抗病性的关系,以抗病湿地松瓶内组培苗和温室1年生组培苗针叶为材料,测定PAL、PPO、SOD的活性。结果表明:松针褐斑病菌毒素处理后,湿地松PAL和PPO活性与抗病性成一定正相关关系,SOD活性与植株抗病性在48 168 h内表现有一定的负相关性。Studying on the relationship between phenylalanine ammonialyase （PAL）, polyphenol oxidase （PPO） , superoxide dismutase （SOD） activities of tissue cultured plantlets of slash pine （Pinus elliottii Engelm. ） and its disease-resistance, the PAL, PPO and SOD activities of the plantlets cultured in bottles and in greenhouse were measured. The result showed that after the plantlets were treated with brown-spot needle blight toxin, the PAL and PPO activities showed a positive correlation into disease resistance, and the SOD activity showed a certain degree of negative correlation at the 48-168 h.

关 键 词：湿地松组培苗 松针褐斑病 苯丙氨酸解氨酶 多酚氧化酶 超氧化物歧化酶 

分 类 号：S791.246[农业科学—林木遗传育种]