牛性机能发育阶段与卵泡、黄体因素对卵母细胞体外成熟及体外受精的影响  

Effects of sexual developmental stages, follicular and Luteal factors on in Vitro maturation and in vitro fertilization of bovine oocytes

作　　者：王艳军[1] 梁冠生[2] 刘健[2] 张嘉保[2] 

机构地区：[1]解放军海军医学研究所科技处动物中心,上海200433 [2]解放军军需大学

出　　处：《黑龙江动物繁殖》2000年第2期4-7,共4页Heilongjiang journal of animal reproduction

摘　　要：利用屠宰母牛卵巢．对来自不同性机能发育阶段母牛的卵巢卵母细胞．不同状态卵巢卵泡的卵母细胞的体外成熟（ＩＶＭ）、体外受精（ＩＶＦ）进行了系列研究。结果表明．来自初情期、性成熟母牛卵巢的卵母细胞受精后的卵裂率（ ７４． ７％和 ８１． ５％）、囊胚率（ ２３．０％和 ２６． ８％）显著高于来自初情期前母牛卵巢卵母细胞的卵裂率（１８．８％）和囊胚率（１．８％），Ｐ＜０．０５；有黄体卵巢的卵母细胞体外受精后的发育能力显著低于无黄体卵巢卵母细胞（卵裂率５８．０％：７９． ９％，囊胚率１３．％：２７．１％，Ｐ＜０．０５）；优势卵泡的有无对卵母细胞的卵裂率和囊胚率无明显影响。In order to improve the clevage rate and the blastocysst rate, we have studied several elements including stage of sexual development, follicles and Luteal factors that influnce in vitro maturation and in vitro fertilization of bovine oocytes. The results are described as follows: There was no difference in the cleavage rate(74. 7% and 81. 5%)and the blastocyst rate (23. 0 and 26. 8%) between ovarien oocytes from pubertal heifers and from matured cows. but all of them were significantly higher than those of oocytes from prepubetal heifers (P<0. 05). The development competence after in maturation of oocytes from ovaries with Corpus Luteal was inferior to that of oocytes from ovaries without Corput Luteal (the cleavage rate was 58. 0% and 79. 9%. the blastocyst rate was 13. 4% and 27. 1%. P<0. 05). There was no difference between the oocytes from ovaries with dominent follicle and from ovaries without dominent follicles.

关 键 词：牛卵泡 黄体 卵母细胞 体外成熟 体外受精 发育 

分 类 号：S823.3[农业科学—畜牧学]