用于谷子分子标记辅助选择的SSR标记筛选  

Selection of SSR Markers for Marker-assisted Breeding of Foxtail Millet

作　　者：王永芳[1] 李海权[1] 李伟[1] 张嘉楠[1] 刁现民[1] 

机构地区：[1]河北省农林科学院谷子研究所,国家谷子改良中心,河北省杂粮研究实验室,河北石家庄050031

出　　处：《河北农业科学》2012年第3期1-4,10,共5页Journal of Hebei Agricultural Sciences

基　　金：河北省高层次人才工程项目(2011055005);河北省财政专项(2012055001);国家科技支撑计划项目(2011BAD06B01-1)

摘　　要：育种材料中多态性分子标记的获得是分子标记辅助选择育种的前提。本研究针对谷子生产中常用的6个育种亲本所组配的15个杂交组合,利用193个谷子SSR标记进行检测,从中筛选出适于各组合育种后代材料鉴定的多态性分子标记,为分子标记辅助选择育种奠定基础。结果表明:有96个标记可用于15个组合的后代材料鉴定,每个组合的多态性标记数为22～72个,表现出极显著差异。其中,春谷品种朝谷12号与其他5个夏谷品种所配组合均表现了很好的多态性,多态性标记数为47～72个;夏谷抗除草剂品种K492与其他育种材料所配组合也表现了较好的多态性,多态性标记47～58个。本研究不仅筛选了1批可用于各组合后代材料鉴定的分子标记,还通过分子标记分析各类型材料间的多态性揭示育种材料的遗传基础,为育种材料的应用提供了指导。Polymorphic markers are essential for marker-assisted breeding. 15 hybrid combinations from 6 parents common used in production were examined using 193 SSR primers of foxtail millet, and the polymorphic markers suitable for the identification of hybrid were screened. The results showed that 96 SSR primers were amplified polymorphic products in all 15 hybrid combinations, which accounted for 49.74% of all the primers examined. The number of polymorphic markers in each hybrid combination showed very significant difference （ from 22 to 72 ） 5 hybrid combinations from spring millet variety Chaogu No. 12 and other 5 summer millet varieties, and 5 hybrid combinations from herbicide resistant summer millet variety K492 and other varieties showed more polymorphic, which indicated that Chaogu No. 12 and K492 were two excellent materials for foxtail millet breeding.

关 键 词：谷子 SSR引物 多态性检测 标记辅助育种 

分 类 号：S515[农业科学—作物学]