亚麻原生质体培养及植株再生  

PLANT REGENERATION FROM PROTOPLAST CULTURE IN FLAX (LINUM USITATISSIMUM)

作　　者：苑志辉 卫志明[2] 徐淑平[1] 孙洪涛 吴昌斌 姬研茹 宋淑敏 于莉 

机构地区：[1]中国科学院上海植物生理研究所,植物分子遗传国家重点实验室,双城市150111 [2]黑龙江省亚麻研究所生物技术育种室,上海200032

出　　处：《实验生物学报》2000年第2期163-169,共7页Acta Biologiae Experimentalis Sinica

基　　金：中国科学院上海植物生理研究所植物分子遗传国家重点实验室资助~~

摘　　要：试验分别采用四个纤维用亚麻(Linumusitatissimum)品种7309,948,Belinka和Viking的无菌苗的茎尖为材料游离原生质体。以萌发10天的无菌苗茎尖游离获得的原生质体得率和活性最高,分别达到1.8×10~6/gFW和85.5%。以V-KM为培养基,采用琼脂岛法培养的原生质体,可在培养3天后发生第一次分裂,10天后统计细胞分裂频率为36%,20天后统计植板率达到5.2%。品种7309和Belin-ka再生的愈伤组织接种在均附加0.6mg/L 6-BA和0.1mg/L NAA的B_5-1和MS_3固体培养基上,都有芽苗分化,并分别获得再生植株。品种Viking和948分别仅分化获得了不定根或叶状体。Shoot protoplasts of four fiber flax (Linum usitatissimum) varieties (7309, 948, Belinka and Viking) were isolated and cultured. The optimal condition for higher protoplast yield 1. 8×10~6/ gFW and activity 85. 5%(c. v. 948)were from 10 day old seedings. Culture in V-KM Agroase-is-land medium led to first divisions after 3 days (c. v. 948),and after twenty days with an efficiency of 36% of divided cells and 5. 2% in plating efficiency. Plant regeneration was obtained in 7309 and Belinka on agar media B5-2,MS3 containing 0. 6mg/L 6-BA and 0.1mg/L NAA. Roots and leaves regeneration were observed in Viking and 948 respectively.

关 键 词：亚麻 原生质体培养 植物再生 纤维作物 

分 类 号：S563.2[农业科学—作物学]