靶向env及LTR基因的siRNA抑制ALV-J复制的研究  

siRNAs Targeted to env and LTR Gene for Inhibition of Avian Leukosis Virus Subgroup J Replication

作　　者：李娇[1] 冯少珍[1] 黄武[1] 吴晓婵[1] 李育豪[1] 曹伟胜[1] 廖明[1] 

机构地区：[1]华南农业大学兽医学院,农业部兽用疫苗创制重点实验室,广东广州510642

出　　处：《中国畜牧兽医》2012年第8期16-19,共4页China Animal Husbandry & Veterinary Medicine

基　　金：NSFC-广东联合基金(U0831002);国家肉鸡产业技术体系项目(nycytx-42-G3-03);广东省自然科学基金项目(S2011010001946;10451064201005432)

摘　　要：试验针对J亚群禽白血病病毒的env及LTR基因,根据它们的保守序列,分别设计合成了4～5对siRNA,并将其克隆至pSilencer 4.1,构建siRNA重组表达质粒。将该质粒转染DF-1细胞6 h后以103TCID50接种ALV-J,利用Real-time RT-PCR在mRNA表达水平检测各重组质粒对病毒复制的影响。结果表明,与阴性对照相比,pSi4.1-env各重组质粒对env基因的抑制效果达到18.15%～63.43%,pSi4.1-LTR各重组质粒的抑制效果则达到19.37%～45.41%。According to the env and LTR gene of avian leukosis virus subgroup J,4 and 5 short hairpin RNAs（shRNAs） targeting their conserved sequences were designed systhessised and cloned into pSilencer 4.1 vector respectively.The recombinant plasmids were transfected into DF-1 cell and infected with 103 TCID50 ALV-J at 6 hours post-transfection,the efficiency of RNA interfering was assayed by Real-time RT-PCR.The results revealed that the recombinant plasmids of pSi4.1-env inhibited virus growth by 18.15% to 63.43% and pSi4.1-LTR by 19.37% to 45.41% compared to negative control.

关 键 词：J亚群禽白血病病毒 ENV基因 LTR基因 SIRNA 

分 类 号：S852.659.3[农业科学—基础兽医学]